Saringosterol Acetate Isolated from Sargassum fusiformis Induces Mitochondrial‐Mediated Apoptosis in MCF‐7 Breast Cancer Cells

• Published in: Chemistry & biodiversity Vol. 19; no. 3; pp. e202100848 - n/a
• Main Authors: Li, Xue, Zhou, Dong‐Yue, Li, Fang‐Tong, Jiang, Yun‐Fei, Dai, Yu‐Lin, Jeon, You‐Jin
• Format: Journal Article
• Language: English
• Published: Switzerland Wiley Subscription Services, Inc 01.03.2022
• Subjects: Acetates - pharmacology; Acetic acid; Algae; anticancer effect; Anticancer properties; Apoptosis; Bioactive compounds; Breast cancer; Breast Neoplasms - drug therapy; Caspase-3; Drug development; Female; Humans; MCF-7 Cells; Mitochondria; mitochondria-mediated pathway; Sargassum; Sargassum fusiformis; saringosterol acetate; Seaweeds; Stigmasterol - analogs & derivatives; Survival; Sargassum fusiformis; mitochondria-mediated pathway; saringosterol acetate; anticancer effect
• ISSN: 1612-1872; 1612-1880
• DOI: 10.1002/cbdv.202100848

Sargassum fusiformis is among the most important edible brown seaweeds in Eastern Asia that contains various bioactive compounds and strong activities. Saringosterol acetate (SA) was successfully isolated from S. fusiformis in our previous research. In this study, SA was investigated for its anticancer effect on MCF‐7 breast cancer cells. SA attenuated the survival rate of MCF‐7 cells with an IC50 value of 63.16±3.6 μg/mL. Staining with Hoechst 33342 demonstrated that SA treatment mediated apoptotic body generation. SA significantly downregulated Bcl‐xL and upregulated Bax, and cleaved PARP, and cleaved caspase 3 in a dose‐dependent manner. Thus, these results suggest that SA induced mitochondria‐mediated apoptosis in MCF‐7 cells, making it a plausible candidate for drug development against breast cancer.