Parental Genome Imbalance Causes Hybrid Seed Lethality as Well as Ovary Abscission in Interspecific and Interploidy Crosses in Nicotiana

• Published in: Frontiers in plant science Vol. 13; p. 899206
• Main Authors: He, Hai, Sadahisa, Kumi, Yokoi, Shuji, Tezuka, Takahiro
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 19.05.2022
• Subjects: auxin treatment; endosperm balance number; hybrid seed lethality; interploidy cross; interspecific cross; ovary abscission; Plant Science; interploidy cross; ovary abscission; auxin treatment; hybrid seed lethality; endosperm balance number; tobacco; interspecific cross
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2022.899206

Enhanced ovary abscission after pollination and hybrid seed lethality result in post-zygotic reproductive isolation in plant interspecific crosses. However, the connection between these barriers remains unclear. Here, we report that an imbalance in parental genomes or endosperm balance number (EBN) causes hybrid seed lethality and ovary abscission in both interspecific and intraspecific-interploidy crosses in the genus . Auxin treatment suppressed ovary abscission, but not hybrid seed lethality, in an interspecific cross between and , suggesting that ovary abscission-related genes are located downstream of those involved in hybrid seed lethality. We performed interploidy crosses among tetraploids, octoploids, and neopolyploids and revealed hybrid seed lethality and ovary abscission in interploid crosses. Furthermore, a higher maternal EBN than paternal EBN caused these barriers, as previously observed in × crosses. Altogether, these results suggest that maternal excess of EBN causes hybrid seed lethality, which in turn leads to ovary abscission through the same mechanism in both interspecific and interploidy crosses.