A Bio‐Inspired Free‐Standing Film with Versatility: From Heterogeneous CTCs Programed Isolating to Breast Cancer Molecular Typing

• Published in: Advanced materials (Weinheim) Vol. 36; no. 9; pp. e2309046 - n/a
• Main Authors: Bai, Mengqi, Qin, Rong, Cheng, Kai, Wang, Zengkai, Zhang, Liwei, Tian, Xiaohua, Zhang, Feiyi, Yang, Yuhe, Hou, Shuai, Xu, Jian, Liu, Lei
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.03.2024
• Subjects: Biocompatibility; Biomarkers; bio‐inspired free‐standing film; Blood; Blood Platelets; Breast cancer; Breast Neoplasms - diagnosis; cancer molecular typing; Covalent bonds; dynamic bioactive biointerface; Female; Fibroins; Glutathione; Growth factors; heterogeneous CTCs; Humans; Molecular Typing; Peptides; programmed isolation; Serum albumin; Silk fibroin; Titanium dioxide; dynamic bioactive biointerface; programmed isolation; bio-inspired free-standing film; cancer molecular typing; heterogeneous CTCs
• ISSN: 0935-9648; 1521-4095
• DOI: 10.1002/adma.202309046

Developing a robust strategy for profiling heterogeneous circular tumor cells specifically, distinguishing the phenotypes of which in blood sample of cancer patient precisely, and releasing them sequentially, is significant for cancer management by liquid biopsy. Herein, a bio‐inspired free‐standing and flexible film composed of TiO2 nanotube and silk fibroin, fabricated with multiply dynamic bioactive surface (TSF/MDBS) by a simple and eco‐friendly way including using polydopamine chemistry and dual dynamic covalent chemistry, is reported. The as‐prepared TSF/MDBS binds specific peptides toward cells with epithelial biomarker and human epithelial growth factor receptor 2 (HER2) biomarker, and antifouling agents bovine serum albumin for obviating platelets and proteins adhering of blood, can capture heterogeneous CTCs with enhanced capability due to the cytocompatible soft film and exquisite surface design, and further release the captured cells as program, by specifically breaking down the covalent bonds in sequence via the action of adding biocompatible molecules fructose and glutathione. By applying the TSF/MDBS, it can be tailored into desired pieces for identifying CTCs with different phenotypes (HER2‐high and HER2‐low) from the unprocessed blood samples of breast cancer patients, and finally profiling these heterogeneous CTCs, to discriminate HER2 positive or negative of breast cancer patients in clinical applications. A free‐standing film composed of titanium dioxide nanotube/silk fibroin is developed by an eco‐friendly way. It is an easy‐to‐use platform for capturing and profiling heterogeneous circular tumor cells (CTCs) effectively and accurately from the unprocessed blood samples of patients, discriminating the CTCs with different surface biomarkers, showing the potential in molecular typing of breast cancer.