Ethoxysanguinarine Induces Apoptosis, Inhibits Metastasis and Sensitizes cells to Docetaxel in Breast Cancer Cells through Inhibition of Hakai

Ethoxysanguinarine (ESG) is a benzophenanthridine alkaloid extracted from plants of Papaveraceae family, such as Macleaya cordata (Willd) R. Br. The anti‐cancer activity of ESG has been rarely reported. In this study, we investigated the anti‐breast cancer effect of ESG and its underlying mechanism....

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Published inChemistry & biodiversity Vol. 20; no. 2; pp. e202200284 - n/a
Main Authors Ma, Liang, Xuan, Xiao‐Jing, Chen, Xue‐Ming, Fan, Ming‐Hui, Liu, Jian, Huang, Guo‐Zheng, Liu, Zi
Format Journal Article
LanguageEnglish
Published Switzerland Wiley Subscription Services, Inc 01.02.2023
Subjects
Antineoplastic Agents - pharmacology
Apoptosis
Breast cancer
Breast Neoplasms - pathology
Caspase
Cell Line, Tumor
Cell migration
Cell Proliferation
Docetaxel
ethoxysanguinarine
Female
Flow cytometry
Hakai
Humans
Metastases
Metastasis
Plant extracts
Signal transduction
breast cancer
docetaxel
ethoxysanguinarine
Hakai
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Summary:Ethoxysanguinarine (ESG) is a benzophenanthridine alkaloid extracted from plants of Papaveraceae family, such as Macleaya cordata (Willd) R. Br. The anti‐cancer activity of ESG has been rarely reported. In this study, we investigated the anti‐breast cancer effect of ESG and its underlying mechanism. MTT assay and flow cytometry analysis showed that ESG inhibited the viability and induced apoptosis in MCF7 and MDA‐MB‐231 human breast cancer cells. Western blot revealed that ESG triggered intrinsic and extrinsic apoptotic pathways, as evidenced by the activation of caspase‐8, caspase‐9 and caspase‐3. ESG attenuated breast cancer cell migration and invasion through Hakai/E‐cadherin/N‐cadherin. Moreover, Hakai knockdown sensitized ESG‐triggered viability and motility inhibition, suggesting that Hakai mediated the anti‐breast cancer effect of ESG. In addition, ESG potentiated the anti‐cancer activity of docetaxel (DTX) in breast cancer cells. Overall, our findings demonstrate that ESG exhibits outstanding pro‐apoptosis and anti‐metastasis effects on breast cancer via a mechanism related to Hakai‐related signaling pathway.
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ISSN:1612-1872
1612-1880
DOI:10.1002/cbdv.202200284