Dimensional Reduction of Metal–Organic Frameworks for Enhanced Cryopreservation of Red Blood Cells

• Published in: Angewandte Chemie International Edition Vol. 62; no. 22; pp. e202217374 - n/a
• Main Authors: Lei, Qi, Sun, Yaqian, Huang, Junda, Liu, Wei, Zhan, Xiaolong, Yin, Wenxiang, Guo, Sishi, Sinelshchikova, Anna, Brinker, C. Jeffrey, He, Zhiyuan, Guo, Jimin, Wuttke, Stefan, Zhu, Wei
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 22.05.2023
• Edition: International ed. in English
• Subjects: Cryopreservation; Cryopreservation - methods; Cryoprotective Agents - chemistry; Cryoprotective Agents - pharmacology; Cryoprotectors; Dimensional Reduction; Erythrocytes; Hafnium; Hafnium - chemistry; Hydrogen bonding; Hydrogen Bonding Matching; Ice; Ice-Inhibition; Metal-organic frameworks; Metal-Organic Frameworks - chemistry; Multilayers; Nanoparticles; Recrystallization; Red Blood Cell Cryopreservation; Reduction (metal working); Thin films; Hydrogen Bonding Matching; Metal-Organic Frameworks; Dimensional Reduction; Red Blood Cell Cryopreservation; Ice-Inhibition
• ISSN: 1433-7851; 1521-3773
• DOI: 10.1002/anie.202217374

To increase the red blood cell (RBC) cryopreservation efficiency by metal–organic frameworks (MOFs), a dimensional reduction approach has been proposed. Namely, 3D MOF nanoparticles are progressively reduced to 2D ultra‐thin metal–organic layers (MOLs). We found that 2D MOLs are beneficial for enhanced interactions of the interfacial hydrogen‐bonded water network and increased utilization of inner ordered structures, due to the higher surface‐to‐volume ratio. Specifically, a series of hafnium (Hf)‐based 2D MOLs with different thicknesses (monolayer to stacked multilayers) and densities of hydrogen bonding sites have been synthesized. Both ice recrystallization inhibition activity (IRI) and RBCs cryopreservation assay confirm the pronounced better IRI activity and excellent cell recovery efficiency (up to ≈63 % at a very low concentration of 0.7 mg mL−1) of thin‐layered Hf‐MOLs compared to their 3D counterparts, thereby verifying the dimensional reduction strategy to improved cryoprotectant behaviors. A dimensional reduction strategy in which 3D metal–organic frameworks (MOFs) are gradually reduced to 2D ultra‐thin, flexible metal–organic layers (MOLs), has been proposed to facilitate their cryopreservation applications. The 2D MOLs displayed superior ice crystal growth inhibition for the enhanced interfacial interaction through structural deformation/stretching and increased inner ordered structures utilization efficiency.