Phytochemical Profile and Biological Activities of Helianthemum canum l. baumg. from Turkey

• Published in: Chemistry & biodiversity Vol. 14; no. 7
• Main Authors: Baldemir, Ayşe, Gökşen, Neslihan, Ildız, Nilay, Karatoprak, Gökçe Şeker, Koşar, Müberra
• Format: Journal Article
• Language: English
• Published: Switzerland Wiley Subscription Services, Inc 01.07.2017
• Subjects: 3,5‐Dihydroxybenzoic acid; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Antibacterial activities; Antioxidant activities; Antioxidants - chemistry; Antioxidants - isolation & purification; Antioxidants - pharmacology; Apiaceae; Bacteria - drug effects; Carotene; Chelation; Cistaceae - chemistry; Diffusion; Dihydroxybenzoic acid; Flavonoids; Flavonols; Helianthemum canum; High-performance liquid chromatography; Hydroxybenzoates - isolation & purification; In vitro methods and tests; Inhibition; Iron; Iron Chelating Agents - chemistry; Iron Chelating Agents - isolation & purification; Iron Chelating Agents - pharmacology; Linoleic acid; Lipid Peroxidation - drug effects; Liquid chromatography; Methanol; Microorganisms; Minimum inhibitory concentration; Oxidation; Peroxidation; Phenolic compounds; Phenols; Phenols - chemistry; Phenols - isolation & purification; Phenols - pharmacology; Phytochemicals - analysis; Phytochemicals - pharmacology; Plant Extracts - chemistry; Resorcinols - isolation & purification; Scavenging; Thiobarbituric acid; Turkey; Helianthemum canum; 3,5-Dihydroxybenzoic acid; Antibacterial activities; Antioxidant activities; Apiaceae
• ISSN: 1612-1872; 1612-1880
• DOI: 10.1002/cbdv.201700052

In the current study, antioxidant, antibacterial activities, and the phenolic compositions of extracts from Helianthemum canum L. Baumg. (Apiaceae) aerial parts were investigated for the first time. The H. canum was extracted with 70% methanol (HCMeOH) and water (HCW). Both extracts were determined by total phenolic contents (3 mg/ml), flavonoids (1.5 mg/ml), flavonols (1.5 mg/ml), qualitative–quantitative compositions, iron (II) chelation activities (0.1 – 5 mg/ml), free radical scavenging activities (DPPH•: 0.01 – 0.6 mg/ml and ABTS+•: 0.125 – 0.5 mg/ml) and the effect upon inhibition of β‐carotene/linoleic acid co‐oxidation (1 mg/ml). The peroxidation level was also determined using the thiobarbituric acid method (0.01 – 1.5 mg/ml). The results of the activity tests given as IC50 values were estimated from non‐linear algorithm and compared with standards. Antibacterial activities of extracts and standards were evaluated against Gram‐negative and ‐positive ten standard strains using disc diffusion and broth microdilution methods. The MIC results (312.5 – 2500 μg/ml) against tested microorganisms varied from 625 to 2500 μg/ml. In HPLC analysis, 3,5‐dihydroxybenzoic acid was found as the main substance in both extracts. These results showed that HCMeOH was richer in phenolic compounds (284.13 ± 0.30 mg GAE/g extract) from HCW (244.55 ± 0.35 mg GAE/g extract). In conclusion, H. canum extracts showed in vitro antibacterial and antioxidant activities.