A Self‐Serviced‐Track 3D DNA Walker for Ultrasensitive Detection of Tumor Exosomes by Glycoprotein Profiling

Sensitive and accurate analysis of low‐concentration of tumor‐derived exosomes (Exos) in biofluids is essential for noninvasive cancer diagnosis but is still challenging due to the lack of high‐sensitive methods with low‐cost and easy‐operation. Herein, exploiting target Exos as a three‐dimensional...

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Published inAngewandte Chemie International Edition Vol. 61; no. 19; pp. e202116932 - n/a
Main Authors Wang, Huizhen, Zeng, Jiahao, Huang, Jin, Cheng, Hong, Chen, Biao, Hu, Xing, He, Xiaoxiao, Zhou, Yue, Wang, Kemin
Format Journal Article
LanguageEnglish
Published Germany Wiley Subscription Services, Inc 02.05.2022
EditionInternational ed. in English
Subjects
3D DNA Walker
Aptamers
Biosensing Techniques
Cancer
Cell culture
Deoxyribonucleic acid
Diagnosis
DNA
DNA - genetics
Exosomes
Glycoproteins
Humans
Medical diagnosis
Neoplasms - diagnosis
Selectivity
Self-Serviced-Track
Tumor Exosomes
Tumors
Ultrasensitive Detection
3D DNA Walker
Tumor Exosomes
Glycoproteins
Ultrasensitive Detection
Self-Serviced-Track
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Summary:Sensitive and accurate analysis of low‐concentration of tumor‐derived exosomes (Exos) in biofluids is essential for noninvasive cancer diagnosis but is still challenging due to the lack of high‐sensitive methods with low‐cost and easy‐operation. Herein, exploiting target Exos as a three‐dimensional (3D) track for the first time, we developed a self‐serviced‐track DNA walker (STDW) for wash‐free detection of tumor Exos using exosomal glycoprotein, which was enabled by split aptamer‐recognition‐initiated autonomous running powered by a catalytic hairpin assembly (CHA). Benefiting from high selectivity and sensitivity of the STDW assay, direct detection of tumor Exos in cell culture medium and serum could also be realized. Furthermore, this method exhibited high accuracy in clinical sample analysis, offering the potential for early cancer diagnosis and postoperative response prediction. Exploiting target exosomes (Exos) as a three‐dimensional (3D) track: A self‐serviced‐track DNA walker (STDW) for wash‐free and highly sensitive detection of tumor Exos is constructed using split aptamer‐recognition‐initiated autonomous walking powered by a catalytic hairpin assembly. This STDW allows the direct detection of tumor Exos in serum and clinical samples.
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ISSN:1433-7851
1521-3773
DOI:10.1002/anie.202116932