Histological and biochemical effects of preventive and therapeutic vascular photobiomodulation on rat muscle injury

• Published in: Journal of biophotonics Vol. 15; no. 5; pp. e202100271 - n/a
• Main Authors: Lopez, Talita Christine Camillo, Malavazzi, Tainá Caroline dos Santos, Rodrigues, Maria Fernanda Setúbal Destro, Bach, Erna Elisabeth, Silva, Daniela Teixeira, Hi, Edgar Matias Bach, França, Cristiane Miranda, Bussadori, Sandra Kalil, Mesquita‐Ferrari, Raquel Agnelli, Fernandes, Kristianne Porta Santos
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY‐VCH Verlag GmbH & Co. KGaA 01.05.2022; Wiley Subscription Services, Inc
• Subjects: Aspartate aminotransferase; Blood vessels; Chronic conditions; Chronic illnesses; Creatine; Creatine kinase; Edema; Inflammation; Injury prevention; Kinases; Lactic acid; Light therapy; low‐level light therapy; Microscopic analysis; muscle injury; muscle repair; Muscles; Myonecrosis; NLIB; photobiomodulation; Serum levels; vascular photobiomodulation; photobiomodulation; muscle injury; vascular photobiomodulation; muscle repair; NLIB; low-level light therapy
• ISSN: 1864-063X; 1864-0648
• DOI: 10.1002/jbio.202100271

The intravascular or transcutaneous application of photobiomodulation (PBM) over blood vessels (vascular photobiomodulation, VPBM) has been used for the treatment of inflammatory and chronic conditions with promising systemic results. This study evaluated the VPBM effects on a model of muscle regeneration after acute injury and compared the outcomes of preventive and therapeutic VPBM. Transcutaneous VPBM was administered over the rat's main tail vein. Serum levels of creatine kinase (CK), aspartate aminotransferase (AST), and lactate were evaluated and muscles were processed for macroscopic and microscopic analysis. Preventive and therapeutic VPBM led to decreased inflammatory infiltrate, edema, and myonecrosis but with an increase in immature muscle fibers. CK, AST, and lactate levels were lower in the groups treated with VPBM (lowest concentrations in preventive VPBM application). Preventive and therapeutic VPBM were capable of exerting a positive effect on acute muscle injury repair, with more accentuated results when preventive VPBM was administered. The intravascular or transcutaneous application of photobiomodulation (PBM) over blood vessels (vascular photobiomodulation, VPBM) has been used for the treatment of inflammatory and chronic conditions with promising systemic results. This study evaluated the VPBM effects on a model of muscle regeneration after acute injury and compared the outcomes of preventive and therapeutic VPBM. Preventive and therapeutic VPBM were capable of exerting a positive effect on acute muscle injury repair, with more accentuated results when preventive VPBM was administered.