In Situ Self‐Assembly of Fluorogenic RNA Nanozipper Enables Real‐Time Imaging of Single Viral mRNA Translation

Real‐time visualization of individual viral mRNA translation activities in live cells is essential to obtain critical details of viral mRNA dynamics and to detect its transient responses to environmental stress. Fluorogenic RNA aptamers are powerful tools for real‐time imaging of mRNA in live cells,...

Full description

Saved in:
Bibliographic Details
Published inAngewandte Chemie International Edition Vol. 62; no. 25; pp. e202217230 - n/a
Main Authors Zhang, Yu‐Peng, Wang, Zhi‐Gang, Tian, Yi‐Fan, Jiang, Lin‐Han, Zhao, Liang, Kong, De‐Ming, Li, Xing, Pang, Dai‐Wen, Liu, Shu‐Lin
Format Journal Article
LanguageEnglish
Published Germany Wiley Subscription Services, Inc 19.06.2023
EditionInternational ed. in English
Subjects
Aptamers
Elongation
Environmental stress
Genetic code
Imaging
mRNA
Nanozippers
Self-assembly
Single mRNA Imaging
Transient response
Translation
Translation elongation
Translation
Nanozippers
Single mRNA Imaging
Aptamers
Online AccessGet full text

Cover

More Information
Summary:Real‐time visualization of individual viral mRNA translation activities in live cells is essential to obtain critical details of viral mRNA dynamics and to detect its transient responses to environmental stress. Fluorogenic RNA aptamers are powerful tools for real‐time imaging of mRNA in live cells, but monitoring the translation activity of individual mRNAs remains a challenge due to their intrinsic photophysical properties. Here, we develop a genetically encoded turn‐on 3,5‐difluoro‐4‐hydroxybenzylidene imidazolinone (DFHBI)‐binding RNA nanozipper with superior brightness and high photostability by in situ self‐assembly of multiple nanozippers along single mRNAs. The nanozipper enables real‐time imaging of the mobility and dynamic translation of individual viral mRNAs in live cells, providing information on the spatial dynamics and translational elongation rate of viral mRNAs. We developed a genetically encoded RNA nanozipper that allows real‐time imaging of the mobility and dynamic translation of individual viral mRNAs by in situ self‐assembly in live cells, providing information on the spatial dynamics and translation elongation rates of viral mRNAs. This can provide an effective method to monitor the translation activity of individual viral mRNAs in live cells.
Bibliography:These authors contributed equally to this work.
ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 14
content type line 23
ISSN:1433-7851
1521-3773
1521-3773
DOI:10.1002/anie.202217230