Colorimetric array sensor based on bimetallic nitrogen-doped carbon-based nanozyme material to detect multiple antioxidants

• Published in: Mikrochimica acta (1966) Vol. 191; no. 7; p. 365
• Main Authors: Chu, Tingting, Liu, Yaopeng, Gao, Yi, Zhou, Chengyu, Huang, Wensheng, Zheng, Yin
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.07.2024; Springer; Springer Nature B.V
• Subjects: Analytical Chemistry; Antioxidants; Antioxidants - analysis; Antioxidants - chemistry; Ascorbic acid; Ascorbic Acid - analysis; Ascorbic Acid - blood; Ascorbic Acid - chemistry; Benzidines - chemistry; Bimetals; Carbon; Carbon - chemistry; Catalysis; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Cobalt - chemistry; Colorimetry; Colorimetry - methods; Copper - chemistry; Cysteine; Cysteine - blood; Cysteine - chemistry; Discriminant analysis; Dopamine; Dopamine - analysis; Dopamine - blood; Dopamine - chemistry; Glutathione; Glutathione - blood; Glutathione - chemistry; Humans; Hydrogen peroxide; Hydrogen Peroxide - chemistry; Limit of Detection; Lysine; Microengineering; Nanochemistry; Nanotechnology; Nitrogen; Nitrogen - chemistry; Original Paper; Oxidation; Oxidation-Reduction; Peroxidase; Polyphenols; Polyphenols - analysis; Polyphenols - chemistry; Pyrolysis; Sensor arrays; Sensors; Uric acid; Uric Acid - analysis; Uric Acid - blood; Uric Acid - chemistry; Nanozyme; Linear discriminant analysis; Antioxidant; Sensor array
• ISSN: 0026-3672; 1436-5073; 1436-5073
• DOI: 10.1007/s00604-024-06444-8

Copper-cobalt bimetallic nitrogen-doped carbon-based nanoenzymatic materials (CuCo@NC) were synthesized using a one-step pyrolysis process. A three-channel colorimetric sensor array was constructed for the detection of seven antioxidants, including cysteine (Cys), uric acid (UA), tea polyphenols (TP), lysine (Lys), ascorbic acid (AA), glutathione (GSH), and dopamine (DA). CuCo@NC with peroxidase activity was used to catalyze the oxidation of TMB by H 2 O 2 at three different ratios of metal sites. The ability of various antioxidants to reduce the oxidation products of TMB (ox TMB) varied, leading to distinct absorbance changes. Linear discriminant analysis (LDA) results showed that the sensor array was capable of detecting seven antioxidants in buffer and serum samples. It could successfully discriminate antioxidants with a minimum concentration of 10 nM. Thus, multifunctional sensor arrays based on CuCo@NC bimetallic nanoenzymes not only offer a promising strategy for identifying various antioxidants but also expand their applications in medical diagnostics and environmental analysis of food. Graphical Abstract