Photochemical activity and the structure of chloroplasts in Arabidopsis thaliana L. mutants deficient in phytochrome A and B

• Published in: Protoplasma Vol. 254; no. 3; pp. 1283 - 1293
• Main Authors: Kreslavski, Vladimir D., Kosobryukhov, Anatoly A., Schmitt, Franz-Josef, Semenova, Galina A., Shirshikova, Galina N., Khudyakova, Aleksandra Yu, Allakhverdiev, Suleyman I.
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.05.2017; Springer Nature B.V
• Subjects: Arabidopsis - genetics; Arabidopsis - metabolism; Biomedical and Life Sciences; Cell Biology; Chloroplasts - metabolism; Chloroplasts - ultrastructure; Cryptochromes - metabolism; Life Sciences; Light; Original Article; Photochemical Processes; Photoreceptors, Plant - metabolism; Photosynthesis - physiology; Photosystem II Protein Complex - metabolism; Phytochrome A - deficiency; Phytochrome A - genetics; Phytochrome B - deficiency; Phytochrome B - genetics; Plant Leaves - metabolism; Plant Sciences; Ultraviolet Rays; Zoology; UV-radiation; Photosystem II; Photosynthetic apparatus; L; Stress resistance; Phytochromes A and B; Arabidopsis thaliana L
• ISSN: 0033-183X; 1615-6102
• DOI: 10.1007/s00709-016-1020-9

The reduced content of photoreceptors, such as phytochromes, can decrease the efficiency of photosynthesis and activity of the photosystem II (PSII). For the confirmation of this hypothesis, the effect of deficiency in both phytochromes (Phy) A and B (double mutant, DM) in 7–27-day-old Arabidopsis thaliana plants on the photosynthetic activity was studied in absence and presence of UV-A radiation as a stress factor. The DM with reduced content of apoproteins of PhyA and PhyB and wild type (WT) plants with were grown in white and red light (WL and RL, respectively) of high (130 μmol quanta m −2 s −1 ) and low (40 μmol quanta m −2 s −1 ) intensity. For DM and WT grown in WL, no notable difference in the photochemical activity of PSII was observed. However, the resistance of the photosynthetic apparatus (PA) to UV-A and the rate of photosynthesis under light saturation were lower in the DM compared to those in the WT. Growth in RL, when the photoreceptors of blue light—cryptochromes—are inactive, resulted in the significant decrease of the photochemical activity of PSII in DM compared to that in WT including amounts of Q B -non-reducing complexes of PSII and noticeable enhancement of thermal dissipation of absorbed light energy. In addition, marked distortion of the thylakoid membrane structure was observed for DM grown in RL. It is suggested that not only PhyA and PhyB but also cryptochromes are necessary for normal functioning of the PA and formation of the mechanisms of its resistance to UV-radiation.