Telomerase activity in breast cancer patients: association with poor prognosis and more aggressive phenotype

• Published in: Medical oncology (Northwood, London, England) Vol. 33; no. 3; p. 23
• Main Authors: Kulić, Ana, Plavetić, Natalija Dedić, Gamulin, Stjepan, Jakić-Razumović, Jasminka, Vrbanec, Damir, Sirotković-Skerlev, Maja
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.03.2016; Springer Nature B.V
• Subjects: Adult; Biomarkers, Tumor - metabolism; Breast cancer; Breast Neoplasms - diagnosis; Breast Neoplasms - enzymology; Breast Neoplasms - surgery; Disease-Free Survival; Enzyme Activation - physiology; Female; Hematology; Humans; Internal Medicine; Medicine; Medicine & Public Health; Middle Aged; Oncology; Original Paper; Pathology; Phenotype; Prognosis; Telomerase - metabolism; Breast cancer; Prognosis; Telomerase activity; Aggressive phenotype
• ISSN: 1357-0560; 1559-131X
• DOI: 10.1007/s12032-016-0736-x

Telomerase expression is an important mechanism of tumor unlimited replicative potential. The aim of this study was to evaluate prognostic impact of telomerase activity in breast cancer patients and to correlate telomerase activity with established prognostic factors. We analyzed tissue of 102 malignant breast lesions and 20 healthy breast tissues. Telomerase activity was determined by telomeric repeat amplification protocol assay. Telomerase activity was present in 77 (75.49 %) of 102 breast cancers. Telomerase activity in breast cancers was statistically significantly higher in comparison with the activity in normal breast tissue. The levels of telomerase activity were significantly positively correlated with tumor size, axillary nodal status, histological grade, HER-2/neu protein expression in tumor tissue and expression of the nuclear antigen Ki-67. A statistically significant negative correlation was found between the presence of ER and telomerase activity. There was no correlation between telomerase activity and concentration of PR or the age of patients. Kaplan–Meier analysis showed that patients with higher telomerase activity had significantly shorter 10-year disease-free survival ( p  < 0.0001) and 10-year overall survival ( p  < 0.0001) than those with lower telomerase activity. These results were confirmed by logistic regression analysis. Our results support the prognostic role of telomerase activity and its relationship with the more aggressive phenotype of breast cancer.