Microbial screening in hydroxylation of l-proline

• Published in: Tetrahedron letters Vol. 47; no. 51; pp. 9073 - 9076
• Main Authors: Bontoux, M.-C., Gelo-Pujic, M.
• Format: Journal Article
• Language: English
• Published: OXFORD Elsevier Ltd 18.12.2006; Elsevier
• Subjects: Chemistry; Chemistry, Organic; Hydroxylation; l-Proline; Microbial screening; Physical Sciences; Science & Technology; Hydroxylation; Microbial screening; l-Proline; microbial screening; 3-HYDROXYLASE; STREPTOMYCES; hydroxylation; CLONING; L-proline

Microbial screening resulted in identification of five strains with the activity of prolyl 4-hydroxylase. All five strains hydroxylated regioselectively and enantioselectively l-proline into 4( R)- trans-hydroxy- l-proline 1. Microbial screening of 250 wild type strains resulted in identification of...