Microbial screening in hydroxylation of l-proline

Microbial screening resulted in identification of five strains with the activity of prolyl 4-hydroxylase. All five strains hydroxylated regioselectively and enantioselectively l-proline into 4( R)- trans-hydroxy- l-proline 1. Microbial screening of 250 wild type strains resulted in identification of...

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Bibliographic Details
Published inTetrahedron letters Vol. 47; no. 51; pp. 9073 - 9076
Main Authors Bontoux, M.-C., Gelo-Pujic, M.
Format Journal Article
LanguageEnglish
Published OXFORD Elsevier Ltd 18.12.2006
Elsevier
Subjects
Chemistry
Chemistry, Organic
Hydroxylation
l-Proline
Microbial screening
Physical Sciences
Science & Technology
Hydroxylation
Microbial screening
l-Proline
microbial screening
3-HYDROXYLASE
STREPTOMYCES
hydroxylation
CLONING
L-proline
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Summary:Microbial screening resulted in identification of five strains with the activity of prolyl 4-hydroxylase. All five strains hydroxylated regioselectively and enantioselectively l-proline into 4( R)- trans-hydroxy- l-proline 1. Microbial screening of 250 wild type strains resulted in identification of five strains with the activity of prolyl hydroxylase. All five strains hydroxylated regioselectively and enantioselectively l-proline into 4( R)- trans-hydroxy- l-proline 1. The best conversions were obtained with a wild type of Aeromonas caviae. 3-Hydroxylase activity was not detected.
ISSN:0040-4039
1873-3581
DOI:10.1016/j.tetlet.2006.10.094