Association between GT-repeat polymorphism at heme oxygenase-1 gene promoter and gastric cancer and metastasis

• Published in: Tumor biology Vol. 36; no. 6; pp. 4757 - 4762
• Main Authors: Motovali-Bashi, M., Hamidy, M.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.06.2015; Springer Nature B.V
• Subjects: Adult; Aged; Biomedical and Life Sciences; Biomedicine; Cancer Research; Dinucleotide Repeats - genetics; Female; Gastric cancer; Genetic Association Studies; Genotype; Genotype & phenotype; Heme Oxygenase-1 - genetics; Humans; Male; Metastasis; Middle Aged; Neoplasm Metastasis; Polymorphism; Promoter Regions, Genetic; Research Article; Risk Factors; Stomach Neoplasms - genetics; Stomach Neoplasms - pathology; Transcription factors; GT-repeat polymorphism; Polyacrylamide PAGE; Metastasis; 1 promoter; Gastric cancer
• ISSN: 1010-4283; 1423-0380
• DOI: 10.1007/s13277-015-3125-8

HO- 1 gene encodes heme oxygenase-1 enzyme that catalyzes the oxidation of heme to carbon monoxide (CO). It has also been suggested that cells could be protected by the enzyme against stress. A (GT) n dinucleotide repeat at HO- 1 promoter is a polymorphic region and modulates gene transcription and associated with some of diseases. In this study, length of polymorphism GT tandem repeat has been determined and classified into two alleles short (≤28) and long (≥29). In present study, association between GT-repeat polymorphism at heme oxygenase -1 gene promoter and increased risk of gastric cancer and metastasis was investigated. Blood samples from 100 control individuals and 60 gastric cancer cases had taken. Genotypic frequencies of (GT) n repeat for samples were determined using PCR technique and polyacrylamide PAGE electrophoresis. At final, higher frequency alleles were sequenced. Our results show that S-allele is significantly higher in cases in comparison with control groups ( p  = 0/000, odds ratio (OR) = 4/154). It has been shown that individuals with S/S and S/L genotypes are at high risk of having gastric cancer ( p  = 0/000, OR = 3/789). Statistic data show association between SS genotype and risk of gastric cancer metastasis ( p  = 0.017, OR = 3.889). But, there is no significant association between clinicopathological characteristics of the patients and risk of gastric cancer metastasis ( p  > 0.05). Significant association was found between short allele (SS + SL genotypes) and risk of gastric cancer, and also strong association was found between SS genotype and risk of gastric cancer metastasis.