SHORT REPORT: SURVEILLANCE OF LEISHMANIA SP. AMONG SAND FLIES IN SICILY (ITALY) USING A FLUOROGENIC REAL-TIME POLYMERASE CHAIN REACTION

Leishmaniasis caused by Leishmania infantum is a complex zoonotic disease, resulting in cutaneous and visceral manifestations in both dogs and humans. The present study involved a published Taqman fluorogenic real-time polymerase chain reaction (PCR) assay for surveillance of Leishmania sp. parasite...

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Published inThe American journal of tropical medicine and hygiene Vol. 72; no. 2; pp. 138 - 141
Main Authors GOMEZ-SALADIN, EDUARDO, DOUD, CARL W, MAROLI, MICHELE
Format Journal Article
LanguageEnglish
Published United States ASTMH 01.02.2005
Subjects
Animals
DNA, Protozoan - analysis
Female
Insect Vectors - parasitology
Leishmania - classification
Leishmania - genetics
Leishmania - isolation & purification
Leishmaniasis - epidemiology
Leishmaniasis - transmission
Male
Polymerase Chain Reaction
Psychodidae - parasitology
Seasons
Sicily - epidemiology
Sicily
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Summary:Leishmaniasis caused by Leishmania infantum is a complex zoonotic disease, resulting in cutaneous and visceral manifestations in both dogs and humans. The present study involved a published Taqman fluorogenic real-time polymerase chain reaction (PCR) assay for surveillance of Leishmania sp. parasites among sand flies trapped in two provinces in Sicily, Catania and Agrigento, during the summer and fall of 2003. Only male specimens were identified to species level, while females were used to evaluate Leishmania sp. infection by PCR testing. The two most prevalent sand fly species found were Phlebotomus perfiliewi and P. perniciosus. Of the female sand flies tested, 2.9% were positive for Leishmania sp. DNA by the PCR.
Bibliography:ObjectType-Article-1
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ISSN:0002-9637
1476-1645
DOI:10.4269/ajtmh.2005.72.138