Drug loaded microbeads entrapped electrospun mat for wound dressing application

• Published in: Journal of materials science. Materials in medicine Vol. 28; no. 6; p. 88
• Main Authors: Sundaran, P. Sneha, Bhaskaran, Aswathy, Alex, Sherrin T., Prasad, Tilak, Haritha, V. H., Anie, Y., Kumary, T. V., Anil Kumar, P. R.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.06.2017; Springer Nature B.V
• Subjects: Agar; Ampicillin; Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibiotics; Antiinfectives and antibacterials; Bandages; Biocompatibility; Biocompatible Materials; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Blood Platelets; Cell adhesion; Cell adhesion & migration; Cell Line; Ceramics; Chemistry and Materials Science; Chitosan; Clinical Applications of Biomaterials; Composites; Cytotoxicity; Drug Carriers; Electrochemical Techniques; Electron microscopy; Electrospinning; Fibroblasts - physiology; Fourier analysis; Fourier transforms; Glass; Hemolysis; Infrared spectroscopy; Materials Science; Materials Testing; Mice; Microspheres; Nanoparticles; Natural Materials; Penicillins - chemistry; Penicillins - pharmacology; Polycaprolactone; Polymer Sciences; Regenerative Medicine/Tissue Engineering; Scanning electron microscopy; Staphylococcus aureus - drug effects; Streptomycin - chemistry; Streptomycin - pharmacology; Surface chemistry; Surfaces and Interfaces; Thin Films; Toxicity; Wound healing; Wounds; Site Specific Drug Delivery; Environmental Scanning Electron Microscopy; Chitosan; Percentage Hemolysis; Chitosan Bead
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-017-5893-8

A new design of antibiotic loaded wound dressing and its initial in vitro evaluation is described. Chitosan microbeads loaded with ampicillin were sandwiched within polycaprolactone electrospun mat (MbAPPCL). The morphology was analyzed by scanning electron microscopy and surface chemistry was characterized by Fourier Transform Infrared Spectroscopy. In vitro cytotoxicity using L-929 fibroblast cells by direct contact test and elution assay revealed non-cytotoxic nature of MbAPPCL. The cell adhesion and viability analysis further confirmed the cytocompatibility of MbAPPCL as a wound dressing material. Percentage hemolysis and platelet adhesion on the mat exposed to blood substantiated the hemocompatibility. The antibiotic susceptibility test analyzed on Staphylococcus aureus by agar plate method confirmed the drug release and antimicrobial property. The proposed wound dressing model explained with ampicillin as a candidate drug has the potential to include microbeads with different antibiotics for multi drug treatment. Graphical Abstract