Paenibacillus maysiensis sp. nov., a Nitrogen-Fixing Species Isolated from the Rhizosphere Soil of Maize

• Published in: Current microbiology Vol. 75; no. 10; pp. 1267 - 1273
• Main Authors: Wang, Tian-shu, Xie, Jiu-yan, Wang, Li-ying, Chen, San-feng
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.10.2018; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biotechnology; Corn; Deoxyribonucleic acid; DNA; Fatty acids; Gene sequencing; Genomes; Hybridization; Life Sciences; Microbiology; New species; NifH gene; Nitrogen fixation; Paenibacillus; Phenotypes; Phylogeny; Proteins; Quinones; Rhizosphere; rRNA 16S; Similarity; Soil analysis
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-018-1519-8

A novel bacterium SX-49 T with nitrogen-fixing capability was isolated from the rhizosphere soil of maize. Phylogenetic analysis of nifH gene fragment and 16S rRNA gene sequence revealed that the strain SX-49 T is a member of the genus Paenibacillus . Values of 16S rRNA gene sequence similarity were highest between SX-49 T and P. jamilae DSM 13815 T (97.0%), P. brasiliensis DSM 14914 T (97.8%), P. polymyxa DSM 36 T (97.5%), and P. terrae DSM 15891 T (98.8%). The similarity between SX-49 T and other Paenibacillus species was < 97.0%. DNA–DNA hybridization values between strain SX-49 T and the four type strains were P. jamilae DSM 13815 T : 40.6%, P. brasiliensis DSM 14914 T : 27.9%, P. polymyxa DSM 36 T : 29.2%, and P. terrae DSM 15891 T : 66.4%. The DNA G+C content of SX-49 T was 46.4 mol%. The predominant fatty acids were anteiso-C 15:0 , C 16:0 and iso-C 16:0 . The predominant isoprenoid quinone was MK-7. The genome contains 5628 putative protein-coding sequences (CDS), 6 rRNAs and 56 tRNAs. The phenotypic and genotypic characteristics, DNA–DNA relatedness, and genome features suggest that SX-49 T represents a novel species of the genus Paenibacillus , and the name Paenibacillus maysiensis sp. nov. is proposed.