Paenibacillus maysiensis sp. nov., a Nitrogen-Fixing Species Isolated from the Rhizosphere Soil of Maize
A novel bacterium SX-49 T with nitrogen-fixing capability was isolated from the rhizosphere soil of maize. Phylogenetic analysis of nifH gene fragment and 16S rRNA gene sequence revealed that the strain SX-49 T is a member of the genus Paenibacillus . Values of 16S rRNA gene sequence similarity were...
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Published in | Current microbiology Vol. 75; no. 10; pp. 1267 - 1273 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.10.2018
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | A novel bacterium SX-49
T
with nitrogen-fixing capability was isolated from the rhizosphere soil of maize. Phylogenetic analysis of
nifH
gene fragment and 16S rRNA gene sequence revealed that the strain SX-49
T
is a member of the genus
Paenibacillus
. Values of 16S rRNA gene sequence similarity were highest between SX-49
T
and
P. jamilae
DSM 13815
T
(97.0%),
P. brasiliensis
DSM 14914
T
(97.8%),
P. polymyxa
DSM 36
T
(97.5%), and
P. terrae
DSM 15891
T
(98.8%). The similarity between SX-49
T
and other
Paenibacillus
species was < 97.0%. DNA–DNA hybridization values between strain SX-49
T
and the four type strains were
P. jamilae
DSM 13815
T
: 40.6%,
P. brasiliensis
DSM 14914
T
: 27.9%,
P. polymyxa
DSM 36
T
: 29.2%, and
P. terrae
DSM 15891
T
: 66.4%. The DNA G+C content of SX-49
T
was 46.4 mol%. The predominant fatty acids were anteiso-C
15:0
, C
16:0
and iso-C
16:0
. The predominant isoprenoid quinone was MK-7. The genome contains 5628 putative protein-coding sequences (CDS), 6 rRNAs and 56 tRNAs. The phenotypic and genotypic characteristics, DNA–DNA relatedness, and genome features suggest that SX-49
T
represents a novel species of the genus
Paenibacillus
, and the name
Paenibacillus maysiensis
sp. nov. is proposed. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0343-8651 1432-0991 |
DOI: | 10.1007/s00284-018-1519-8 |