Obtaining phenolic compounds from jatoba (Hymenaea courbaril L.) bark by supercritical fluid extraction

• Published in: The Journal of supercritical fluids Vol. 89; pp. 68 - 77
• Main Authors: Veggi, Priscilla C., Prado, Juliana M., Bataglion, Giovana A., Eberlin, Marcos N., Meireles, M. Angela A.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.05.2014
• Subjects: Antioxidant activity; Cosolvents; Phenolic compounds; Supercritical fluid extraction; Tannins; Tannins; Cosolvents; Phenolic compounds; Supercritical fluid extraction; Antioxidant activity
• ISSN: 0896-8446; 1872-8162
• DOI: 10.1016/j.supflu.2014.02.016

[Display omitted] •The highest yield obtained for jatoba extract was 24%, using CO2 and water.•A 30-fold scale-up to pilot scale was performed for supercritical process.•The maximum total phenolic compounds and total tannins content were 335.00mg TAE/g extract (d.b.), and 1.8g/100g raw material, respectively. The extraction of polyphenol compounds from jatoba (Hymenaea courbaril L. var stilbocarpa) bark using supercritical fluid extraction (SFE) with CO2 and cosolvents has been investigated. Among the solvent systems studied, SFE using CO2 and water (9:1, v/v), at 323K and 35MPa, presented the best results, with extract yield of 24%, and with high antioxidant activity (IC50 of 0.2mg/cm3). This solvent system was used to determine global yield isotherms, which were built at 323 and 333K, and 15, 25, and 35MPa, using a second lot of jatoba. The highest yield was 11.5% at 15MPa and 323K, with maximum total phenolic compounds (TPC) of 335.00mg TAE/g extract (d.b.) and total tannins content of 1.8g/100g raw material. A kinetic experiment was performed using optimized conditions, yielding 18% extract, and the kinetic parameters were used to scale-up the process from laboratory to pilot scale. Chemical analyses showed high content of phenolic compounds in the extracts of jatoba bark mostly due to the presence of procyanidins.