Association Between MMP8 Gene Polymorphisms and Laryngeal Squamous Cell Carcinoma

• Published in: Anticancer research Vol. 40; no. 4; pp. 2003 - 2009
• Main Authors: Insodaite, Ruta, Liutkevicius, Vykintas, Uloza, Virgilijus, Solovejute, Rosita, Smalinskiene, Alina
• Format: Journal Article
• Language: English
• Published: Greece International Institute of Anticancer Research 01.04.2020
• Subjects: Alleles; Anticancer properties; Carcinoma, Squamous Cell - genetics; Carcinoma, Squamous Cell - pathology; Confidence; Control methods; Deoxyribonucleic acid; DNA; Embryogenesis; Embryonic growth stage; Extracellular matrix; Female; Genetic Association Studies; Genetic Predisposition to Disease; Genotype; Humans; Laryngeal cancer; Laryngeal Neoplasms - genetics; Laryngeal Neoplasms - pathology; Male; Matrix metalloproteinase; Matrix Metalloproteinase 8 - genetics; Matrix metalloproteinases; Middle Aged; Neutrophil collagenase; Nucleotides; Polymerase chain reaction; Polymorphism, Single Nucleotide; Regression analysis; Single-nucleotide polymorphism; Smoking; Squamous cell carcinoma; Statistical analysis; Statistical methods; Tumors; matrix metalloproteinase 8; MMR8; rs11225395; rs2155052; Laryngeal squamous cell carcinoma; single nucleotide polymorphism; matrix metalloproteinases
• ISSN: 0250-7005; 1791-7530
• DOI: 10.21873/anticanres.14156

Matrix metalloproteinases (MMPs) are a family of proteins which are involved in breakdown of the extracellular matrix in embryonic development, tissue remodeling and in some diseases. MMP8 has both cancer-promoting and anticancer properties. However, the contribution of MMP8 to laryngeal squamous cell carcinoma (LSCC) has not been elucidated. In this study we aimed to test the contribution of two MMP8 polymorphisms, located in the gene promoter region, to the development of LSCC. This case-control study involved 569 DNA samples which were genotyped for two single nucleotide polymorphisms using real-time polymerase chain reaction method. Statistical analysis was performed with SPSS Statistics 20 software. Regression analysis adjusted by age showed that for MMP8 rs11225395 each minor A allele copy significantly reduced the odds for LSCC development (odds ratio=0.49, 95% confidence intervaI=0.04-2.19, p=0.048). MMP8 rs11225395 AA genotype was associated with smaller laryngeal tumour size (p=0.023). Smoking habit also correlated with laryngeal tumor size. MMP8 rs11225395 and smoking habits have a prominent interface with LSCC tumour size.