Sphingobacterium praediipecoris sp. nov. isolated from effluent of a dairy manure treatment plant

• Published in: Archives of microbiology Vol. 200; no. 10; pp. 1481 - 1486
• Main Authors: Van Le, Ve, Padakandla, Shalem Raj, Kim, Hyangmi, Chae, Jong-Chan
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.12.2018; Springer Nature B.V
• Subjects: Antibiotics; Archives & records; Biochemistry; Biomedical and Life Sciences; Biotechnology; Cell Biology; Deoxyribonucleic acid; DNA; Ecology; Effluents; Fatty acids; Life Sciences; Lipids; Manures; Microbial Ecology; Microbiology; Microorganisms; Morphology; New species; Original Paper; pH effects; Phosphatidylethanolamine; Phylogenetics; Phylogeny; Quinones; rRNA 16S; Sodium chloride; Polyphasic characterization; Dairy manure treatment plant; Sphingophospholipid; Sphingobacterium praediipecoris
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-018-1566-3

A novel Gram-reaction-negative, rod-shaped, non-motile bacterium, designated as strain G2-10 T was isolated from effluent of a dairy manure treatment plant. Growth occurred at 20–40 °C (optimum at 25–30 °C), pH 7.0–8.0 (optimum at pH 7.0). The range of NaCl concentration for growth was between 0% and 3% (w/v) (optimum 0–1%, w/v). Comparison of 16S rRNA gene sequence indicated that strain G2-10 T was moderately related to the type strains of Sphingobacterium nematocida M-SX103 T and Sphingobacterium suaedae T47 T with a pair-wise sequence similarity of 94.3% and 94.0%, respectively. The major fatty acid constituents of strain G2-10 T were identified as iso-C 15:0 (37.6%), summed feature 3 (consisting of C 16:1 ω 7c and/or C 16:1 ω 6c, 29.6%) and iso-C 17:0 3-OH (15.2%). Phosphatidylethanolamine was the major polar lipids of strain G2-10 T . Sphingophospholipids were present. The isoprenoid quinone was composed of only MK-7. The DNA G + C content of strain G2-10 T was found to be 42.5 mol%. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain G2-10 T represents a novel species within the genus Sphingobacterium , for which the name Sphingobacterium praediipecoris is proposed. The type strain is G2-10 T (= KCTC 52880 T  = NBRC 112848 T ).