LTR sequence of the MDG4 retrotransposon contains the MAD protein binding site that affects the east-dependent repression

• Published in: Doklady. Biochemistry and biophysics Vol. 473; no. 1; pp. 106 - 110
• Main Authors: Golovnin, A. K., Molodina, V. V., Shapovalov, I. S., Georgiev, P. G., Melnikova, L. S.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.03.2017; Springer Nature B.V
• Subjects: Animals; Base Sequence; Binding Sites; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Bone morphogenetic proteins; Bristles; Chromatin; DNA-Binding Proteins - metabolism; Drosophila melanogaster - genetics; Drosophila melanogaster - metabolism; Drosophila Proteins - metabolism; EAST protein; Gene expression; Gene silencing; Inverted repeat; Life Sciences; Long terminal repeat; MAD protein; Molecular Biology; Proteins; Retroelements - genetics; Terminal Repeat Sequences - genetics; Transcription factors; Transcription Factors - metabolism; Transcription, Genetic - genetics; Yellow gene
• ISSN: 1607-6729; 1608-3091
• DOI: 10.1134/S1607672917020053

Earlier, we showed in a model system of the yellow gene of D. melanogaster that an increase in the EAST protein concentration leads to repression in bristles, the mechanism of which remained obscure. In this study, an inverted repeat was localized by genetic methods in the long terminal repeat (LTR) sequence of the MDG4 retrotransposon. This repeat includes the binding site for the Mad protein—the key component of the of TGF-β/BMP signaling cascade. The results of this work suggest that the Mad protein recruits to chromatin a regulatory complex that functionally interacts with the EAST protein. This complex either itself suppresses the yellow gene expression in bristles or moves the transgene to the nuclear regions with a high concentration of transcription repression factors.