TubStain: a universal peptide-tool to label microtubules

Imaging of the microtubular network is an important strategy to define cell-cycle specific and pathological states of eukaryotic cells. Here, we describe TubStain, a novel recombinant polypeptide allowing direct, non-antibody dependent labeling of microtubules in fixed cells over a broad range of di...

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Bibliographic Details
Published inHistochemistry and cell biology Vol. 138; no. 3; pp. 531 - 540
Main Authors Theiss, Carsten, Neuhaus, Alexander, Schliebs, Wolfgang, Erdmann, Ralf
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer-Verlag 01.09.2012
Springer Nature B.V
Subjects
Amino Acid Sequence
Animals
Biochemistry
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell Division
Cellular biology
Developmental Biology
Eukaryotes
Fibroblasts - metabolism
Humans
Immunohistochemistry
Membrane Proteins - chemistry
Membrane Proteins - metabolism
Microtubules
Molecular Sequence Data
Pathology
Peptides - chemistry
Peptides - metabolism
Polypeptides
Protein Engineering
Rats
Recombinant Fusion Proteins - chemistry
Repressor Proteins - chemistry
Repressor Proteins - metabolism
Short Communications
Tubulin - chemistry
Tubulin - metabolism
Immunohistochemistry
Fluorophore
Cytochemistry
Microtubules
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Summary:Imaging of the microtubular network is an important strategy to define cell-cycle specific and pathological states of eukaryotic cells. Here, we describe TubStain, a novel recombinant polypeptide allowing direct, non-antibody dependent labeling of microtubules in fixed cells over a broad range of different species and tissues. TubStain has, due to its small size and susceptibility for biochemical and genetic manipulations, high potential as a microtubule marker in state-of-the-art microscopy.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0948-6143
1432-119X
DOI:10.1007/s00418-012-0992-6