Cellular Localization of Acid-Sensing Ion Channel 1 in Rat Nucleus Tractus Solitarii

• Published in: Cellular and molecular neurobiology Vol. 38; no. 1; pp. 219 - 232
• Main Authors: Lin, Li-Hsien, Jones, Susan, Talman, William T.
• Format: Journal Article
• Language: English
• Published: New York Springer US 2018; Springer Nature B.V
• Subjects: Acid Sensing Ion Channels - analysis; Acid Sensing Ion Channels - metabolism; Acidosis; Animals; Area postrema; Astrocytes; Astrocytes - chemistry; Astrocytes - metabolism; Biomedical and Life Sciences; Biomedicine; Cell Biology; Confocal microscopy; Glial fibrillary acidic protein; Gliosis; Immunohistochemistry; Injection; Localization; Male; Microglia; Microglia - chemistry; Microglia - metabolism; Microinjection; Motor nuclei; Neurobiology; Neurosciences; Original Research; Protein gene product 9.5; Rats; Rats, Sprague-Dawley; Rodents; Saporin; Solitary Nucleus - chemistry; Solitary Nucleus - cytology; Solitary Nucleus - metabolism; Solitary tract nucleus; Confocal microscopy; Immunofluorescent histochemistry; Astrocyte; Microglia
• ISSN: 0272-4340; 1573-6830; 1573-6830
• DOI: 10.1007/s10571-017-0534-9

By determining its cellular localization in the nucleus tractus solitarii (NTS), we sought anatomical support for a putative physiological role for acid-sensing ion channel Type 1 (ASIC1) in chemosensitivity. Further, we sought to determine the effect of a lesion that produces gliosis in the area. In rats, we studied ASIC1 expression in control tissue with that in tissue with gliosis, which is associated with acidosis, after saporin lesions. We hypothesized that saporin would increase ASIC1 expression in areas of gliosis. Using fluorescent immunohistochemistry and confocal microscopy, we found that cells and processes containing ASIC1-immunoreactivity (IR) were present in the NTS, the dorsal motor nucleus of vagus, and the area postrema. In control tissue, ASIC1-IR predominantly colocalized with IR for the astrocyte marker, glial fibrillary acidic protein (GFAP), or the microglial marker, integrin αM (OX42). The subpostremal NTS was the only NTS region where neurons, identified by protein gene product 9.5 (PGP9.5), contained ASIC1-IR. ASIC1-IR increased significantly (157 ± 8.6% of control, p  < 0.001) in the NTS seven days after microinjection of saporin. As we reported previously, GFAP-IR was decreased in the center of the saporin injection site, but GFAP-IR was increased in the surrounding areas where OX42-IR, indicative of activated microglia, was also increased. The over-expressed ASIC1-IR colocalized with GFAP-IR and OX42-IR in those reactive astrocytes and microglia. Our results support the hypothesis that ASIC1 would be increased in activated microglia and in reactive astrocytes after injection of saporin into the NTS.