tLyP-1-conjugated core-shell nanoparticles, Fe3O4NPs@mSiO2, for tumor-targeted drug delivery

• Published in: Applied surface science Vol. 474; pp. 17 - 24
• Main Authors: Morita, Yasuyuki, Sakurai, Ryohei, Wakimoto, Takuma, Kobayashi, Koudai, Xu, Baiyao, Toku, Yuhki, Song, Guanbin, Luo, Qing, Ju, Yang
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 30.04.2019
• Subjects: Cancer therapy; Core-shell nanoparticles; Hyperthermia; tLyP-1; Tumor-targeted DDS; Core-shell nanoparticles; tLyP-1; Tumor-targeted DDS; Hyperthermia; Cancer therapy
• ISSN: 0169-4332; 1873-5584
• DOI: 10.1016/j.apsusc.2018.09.205

[Display omitted] •tLyP-1-conjugated Fe3O4NPs@mSiO2 core-shell nanoparticles were developed.•The drug delivery nanoparticles have capabilities of targeted therapy and hyperthermia for cancer.•The nanoparticles fabricated reduce viability of tumor cells (Hela) less than 18.3%. Tumor-targeted core-shell nanoparticles were developed for a multifunctional drug delivery system (DDS) for cancer therapy. Fe3O4 nanoparticles with mesoporous silica (Fe3O4NPs@mSiO2), in which Fe3O4 and mSiO2 form the core and shell, respectively, were functionalized to deliver a hydrophobic anti-tumor drug and to heat targeted tumor cells with the energy of an alternating magnetic field. For targeting tumor cells, tLyP-1, a tumor cell homing and penetrating peptide, was engrafted on to the Fe3O4NPs@mSiO2 (Fe3O4NPs@mSiO2-tLyP-1). The fabricated Fe3O4NPs@mSiO2-tLyP-1 were loaded with camptothecin (CPT); they showed robust, selective targeting and penetrating efficacy for Hela cells, and induced cell death. The CPT-loaded Fe3O4NPs@mSiO2-tLyP-1 (Fe3O4NPs@mSiO2-tLyP-1(CPT)) reduced the cell viability of Hela cells to 28.8%. Furthermore, in combination with application of an alternating magnetic field to cause hyperthermia, the nanoparticles made it possible to decrease viability to 18.3%. The systemic toxicity of Fe3O4NPs@mSiO2-tLyP-1(CPT) to human mesenchymal stem cells (hMSCs) was minimal, because the nanoparticles selectively targeted and penetrated the tumor cells. This study indicates that the developed Fe3O4NPs@mSiO2-tLyP-1 have potential as a DDS in the chemo-hyperthermic treatment of cancer.