Semi-quantitative surface enhanced Raman scattering spectroscopic creatinine measurement in human urine samples

This paper presents the development of a semi-quantitative method of measuring the creatinine biomolecule in human urine by the surface enhanced Raman scattering (SERS) technique. Creatinine is one of the major components of urine and can be used to represent the metabolic and renal function of the...

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Published inOptical and quantum electronics Vol. 37; no. 13-15; pp. 1415 - 1422
Main Authors WANG, Tsuei-Lian, HUIHUA KENNY CHIANG, LU, Hui-Hsin, PENG, Fang-Ying
Format Conference Proceeding Journal Article
LanguageEnglish
Published Dordrecht Springer 01.12.2005
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Abstract This paper presents the development of a semi-quantitative method of measuring the creatinine biomolecule in human urine by the surface enhanced Raman scattering (SERS) technique. Creatinine is one of the major components of urine and can be used to represent the metabolic and renal function of the human body. The Raman signal of creatinine is enhanced by 50 nm Au nanoparticles. Raman spectra between 1400 and 1500 cm were analyzed to obtain the relationship between the SERS band area and creatinine concentration. The square of the correlation coefficient is 0.99 in artificial urine over the creatinine range 38.4-153.6 mg/dl. In a human urine experiment, a good linear correlation is observed over the creatinine concentration range 2.56-6.4 mg/dl. The square of correlation coefficient is 0.96.
AbstractList This paper presents the development of a semi-quantitative method of measuring the creatinine biomolecule in human urine by the surface enhanced Raman scattering (SERS) technique. Creatinine is one of the major components of urine and can be used to represent the metabolic and renal function of the human body. The Raman signal of creatinine is enhanced by 50 nm Au nanoparticles. Raman spectra between 1400 and 1500 cm were analyzed to obtain the relationship between the SERS band area and creatinine concentration. The square of the correlation coefficient is 0.99 in artificial urine over the creatinine range 38.4-153.6 mg/dl. In a human urine experiment, a good linear correlation is observed over the creatinine concentration range 2.56-6.4 mg/dl. The square of correlation coefficient is 0.96.
Author PENG, Fang-Ying
HUIHUA KENNY CHIANG
WANG, Tsuei-Lian
LU, Hui-Hsin
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  surname: HUIHUA KENNY CHIANG
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  givenname: Fang-Ying
  surname: PENG
  fullname: PENG, Fang-Ying
  organization: AND FANG-YING PENG Institute of Biomedical Engineering, National Yang-Ming University, No. 155, Sec. 2, Li-Nong St, 112, Taipei, Taiwan, Province of China
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Issue 13-15
Keywords Creatinine
Human
Urine
Experimental device
Experimental study
Colloid
Gold chloride
Organic molecule
SERS
Surface Enhanced Raman Spectrometry
Raman
Au colloid
Helium-Neon lasers
Quantitative analysis
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Snippet This paper presents the development of a semi-quantitative method of measuring the creatinine biomolecule in human urine by the surface enhanced Raman...
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SubjectTerms Biological and medical sciences
Fundamental and applied biological sciences. Psychology
General aspects, investigation technics, apparatus
Tissues, organs and organisms biophysics
Title Semi-quantitative surface enhanced Raman scattering spectroscopic creatinine measurement in human urine samples
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