Semi-quantitative surface enhanced Raman scattering spectroscopic creatinine measurement in human urine samples
This paper presents the development of a semi-quantitative method of measuring the creatinine biomolecule in human urine by the surface enhanced Raman scattering (SERS) technique. Creatinine is one of the major components of urine and can be used to represent the metabolic and renal function of the...
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| Published in | Optical and quantum electronics Vol. 37; no. 13-15; pp. 1415 - 1422 |
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| Main Authors | , , , |
| Format | Conference Proceeding Journal Article |
| Language | English |
| Published |
Dordrecht
Springer
01.12.2005
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| Subjects | |
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| Abstract | This paper presents the development of a semi-quantitative method of measuring the creatinine biomolecule in human urine by the surface enhanced Raman scattering (SERS) technique. Creatinine is one of the major components of urine and can be used to represent the metabolic and renal function of the human body. The Raman signal of creatinine is enhanced by 50 nm Au nanoparticles. Raman spectra between 1400 and 1500 cm were analyzed to obtain the relationship between the SERS band area and creatinine concentration. The square of the correlation coefficient is 0.99 in artificial urine over the creatinine range 38.4-153.6 mg/dl. In a human urine experiment, a good linear correlation is observed over the creatinine concentration range 2.56-6.4 mg/dl. The square of correlation coefficient is 0.96. |
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| AbstractList | This paper presents the development of a semi-quantitative method of measuring the creatinine biomolecule in human urine by the surface enhanced Raman scattering (SERS) technique. Creatinine is one of the major components of urine and can be used to represent the metabolic and renal function of the human body. The Raman signal of creatinine is enhanced by 50 nm Au nanoparticles. Raman spectra between 1400 and 1500 cm were analyzed to obtain the relationship between the SERS band area and creatinine concentration. The square of the correlation coefficient is 0.99 in artificial urine over the creatinine range 38.4-153.6 mg/dl. In a human urine experiment, a good linear correlation is observed over the creatinine concentration range 2.56-6.4 mg/dl. The square of correlation coefficient is 0.96. |
| Author | PENG, Fang-Ying HUIHUA KENNY CHIANG WANG, Tsuei-Lian LU, Hui-Hsin |
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| Cites_doi | 10.1016/S0924-2031(02)00034-6 10.1002/0471721646 10.1088/0953-8984/14/18/202 10.1021/ac035134k 10.1366/0003702981943059 10.1016/S1386-1425(03)00065-9 10.1016/0924-2031(96)00036-7 10.1021/ja028255v 10.1016/S0165-9936(98)00069-7 10.1016/j.jcis.2004.04.030 10.1366/0003702971940468 10.1021/cr980133r 10.1016/S0924-2031(97)00008-8 10.1366/000370203321666533 10.1002/lsm.1058 |
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| Keywords | Creatinine Human Urine Experimental device Experimental study Colloid Gold chloride Organic molecule SERS Surface Enhanced Raman Spectrometry Raman Au colloid Helium-Neon lasers Quantitative analysis |
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| Title | Semi-quantitative surface enhanced Raman scattering spectroscopic creatinine measurement in human urine samples |
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