Semi-quantitative surface enhanced Raman scattering spectroscopic creatinine measurement in human urine samples

• Published in: Optical and quantum electronics Vol. 37; no. 13-15; pp. 1415 - 1422
• Main Authors: WANG, Tsuei-Lian, HUIHUA KENNY CHIANG, LU, Hui-Hsin, PENG, Fang-Ying
• Format: Conference Proceeding Journal Article
• Language: English
• Published: Dordrecht Springer 01.12.2005
• Subjects: Biological and medical sciences; Fundamental and applied biological sciences. Psychology; General aspects, investigation technics, apparatus; Tissues, organs and organisms biophysics; Creatinine; Human; Urine; Experimental device; Experimental study; Colloid; Gold chloride; Organic molecule; SERS; Surface Enhanced Raman Spectrometry; Raman; Au colloid; Helium-Neon lasers; Quantitative analysis
• ISSN: 0306-8919; 1572-817X
• DOI: 10.1007/s11082-005-4221-6

This paper presents the development of a semi-quantitative method of measuring the creatinine biomolecule in human urine by the surface enhanced Raman scattering (SERS) technique. Creatinine is one of the major components of urine and can be used to represent the metabolic and renal function of the human body. The Raman signal of creatinine is enhanced by 50 nm Au nanoparticles. Raman spectra between 1400 and 1500 cm were analyzed to obtain the relationship between the SERS band area and creatinine concentration. The square of the correlation coefficient is 0.99 in artificial urine over the creatinine range 38.4-153.6 mg/dl. In a human urine experiment, a good linear correlation is observed over the creatinine concentration range 2.56-6.4 mg/dl. The square of correlation coefficient is 0.96.