Better understanding of acute gouty attack using CT perfusion in a rabbit model

• Published in: European radiology Vol. 29; no. 6; pp. 3308 - 3316
• Main Authors: Hu, Yabin, Yang, Qing, Gao, Yanyan, Guo, Xuexin, Liu, Yongjian, Li, Can, Du, Yanmeng, Gao, Lei, Sun, Dezheng, Zhu, Congcong, Yan, Mi
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.06.2019; Springer Nature B.V
• Subjects: Animals; Arthritis; Arthritis, Gouty - diagnostic imaging; Arthritis, Gouty - physiopathology; Blood flow; Blood Volume; Change detection; Crystals; Diagnostic Radiology; Disease Models, Animal; Experimental; Hemodynamics; Imaging; Injection; Internal Medicine; Interventional Radiology; Knee; Male; Mathematical models; Medicine; Medicine & Public Health; Microcirculation - physiology; Neovascularization, Pathologic; Neuroradiology; Osteoarthritis, Knee - diagnostic imaging; Osteoarthritis, Knee - physiopathology; Parameters; Perfusion; Rabbits; Radiology; Random Allocation; Regional Blood Flow; Regression analysis; Tomography, X-Ray Computed - methods; Ultrasound; Uric acid; Rabbits; Arthritis, gouty; Perfusion imaging; Multidetector computed tomography
• ISSN: 0938-7994; 1432-1084
• DOI: 10.1007/s00330-018-5871-7

Objective To assess hemodynamic changes related to acute gouty knee arthritis in a rabbit with CT perfusion (CTP) Methods Forty-two rabbits were randomly separated into two groups: the treated group of 30 and the control group of 12. The right knee was injected with monosodium urate solution and polymyxin in the treated group and saline and polymyxin in the control group. At 2, 16, 32, 48, 60, and 72 h after injection, five rabbits from the treated group and two rabbits from the control group were selected for CTP. At each time point, blood flow (BF), blood volume (BV), and clearance rate (CL) were measured, and microvessel density (MVD) was evaluated with a microscope. Results In the treated group, BF, BV, CL, and MVD were significantly higher than in the control group ( p  < 0.001). Differences within paired comparison of BV, BF, CL, and MVD were all significant (all p  < 0.001). Peak time of BV, BF, and MVD was 32 h and 48 h for CL. After multivariate stepwise linear regression analysis, BV was linearly associated with MVD and vice versa, which also applied to BF with MVD and BF with CL, separately. The ascending rate of MVD was the highest among that of all parameters; so was the descending rate of CL. Conclusion CTP in this rabbit knee model accurately detected hemodynamic changes during a gouty attack. Key Points • Acute gouty arthritis can be evaluated with CTP in a rabbit knee model. • Following injection of MSU crystals, producing an acute gouty attack, CTP successfully assessed hemodynamic changes. • The ascending rate of MVD was the highest among that of all parameters; so was the descending rate of CL.