Neonatal Lethality and Inflammatory Phenotype of the New Transgenic Mice with Overexpression of Human Interleukin-6 in Myeloid Cells

• Published in: Doklady. Biochemistry and biophysics Vol. 483; no. 1; pp. 344 - 347
• Main Authors: Zvartsev, R. V., Korshunova, D. S., Gorshkova, E. A., Nosenko, M. A., Korneev, K. V., Maksimenko, O. G., Korobko, I. V., Kuprash, D. V., Drutskaya, M. S., Nedospasov, S. A., Deikin, A. V.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.11.2018; Springer Nature B.V
• Subjects: Animals; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Bone marrow; Cell culture; Cytokines; Green Fluorescent Proteins - biosynthesis; Green Fluorescent Proteins - genetics; Hematopoiesis; Humans; Inflammation; Interleukin 6; Interleukin-6 - biosynthesis; Interleukin-6 - genetics; Lethality; Life Sciences; Macrophages; Macrophages - cytology; Macrophages - metabolism; Mice; Mice, Transgenic; Molecular Biology; Monocytes; Monocytes - cytology; Monocytes - metabolism; Myeloid cells; Neonates; Phenotypes; Rodents; Transgenic mice
• ISSN: 1607-6729; 1608-3091
• DOI: 10.1134/S1607672918060157

To model human interleukin-6 (hIL-6) associated diseases, unique mice with transgenic overexpression of human IL-6 and reporter fluorescent protein EGFP in cells of macrophage-monocyte lineage were generated using loxP–Cre system. High level of hIL-6 production by macrophages and monocytes, as confirmed in vitro in primary culture of bone marrow-derived macrophages, in vivo resulted in early postnatal death in vivo, presumably, due to the effect of overexpression of hIL-6 on hematopoiesis.