Genetic Variations of VEGFA Gene Are Associated With Infiltration of Adjacent Tissues and the Clinical Outcome of Patients With Nasopharyngeal Carcinoma

• Published in: Anticancer research Vol. 40; no. 2; pp. 677 - 688
• Main Authors: Psoma, Elizabeth, Koliou, Georgia-Angeliki, Dimitrakopoulos, Foteinos-Ioannis, Papadopoulou, Kyriaki, Rontogianni, Dimitra, Bobos, Mattheos, Visvikis, Anastasios, Kosmidis, Paris A, Fountzilas, George, Constantinidis, Jannis, Kalogera-Fountzila, Anna
• Format: Journal Article
• Language: English
• Published: Greece International Institute of Anticancer Research 01.02.2020
• Subjects: Adolescent; Adult; Aged; Cell death; Cell surface; Cohort Studies; Deoxyribonucleic acid; DNA; Endothelins; Female; Genetic diversity; Genetic Predisposition to Disease; Genetic Variation; Genotyping; Growth factors; Humans; Infiltration; Male; Metastases; Middle Aged; Muscles; Nasopharyngeal carcinoma; Nasopharyngeal Carcinoma - genetics; Nasopharyngeal Carcinoma - pathology; Nasopharyngeal Neoplasms - genetics; Nasopharyngeal Neoplasms - pathology; Nucleotides; Peripheral blood; Plates; Polymorphism, Single Nucleotide; Retrospective Studies; Single-nucleotide polymorphism; Throat cancer; Tumors; Vascular endothelial growth factor; Vascular Endothelial Growth Factor A - genetics; Young Adult; CT; EDNRA NBS1; MRI; variations; SNPs; FAS; Nasopharyngeal; cancer; polymorphism; VEGFA; genotype
• ISSN: 0250-7005; 1791-7530
• DOI: 10.21873/anticanres.13997

The aim of the present study was to investigate the clinical significance of 7 single nucleotide polymorphisms (SNPs) of vascular endothelial growth factor A (VEGFA), endothelin receptor type A (EDNRA), nibrin (NBS1) and Fas cell surface death receptor (FAS) genes in patients with nasopharyngeal carcinoma (NPC). Genomic DNA was extracted from the peripheral blood specimens of 60 patients. Genotyping of 4 VEGFA polymorphisms, namely VEGFA -1154 G/A (rs1570360), -2578 A/C (rs699947), -1498 C/T (rs833061) and +936 C/T (rs3025039), as well as EDNRA SNP p.His323 (rs5333), NBS1 p.E185Q (rs1805794) and FAS -671 A/G (rs1800682) was performed by using Sanger sequencing. The VEGFA +936 CC genotype was more frequent in tumors with bilateral infiltration of pterygoid plates compared to those with ipsilateral (76.9% vs. 69.6%, p=0.008) and no infiltration of pterygoid plates (76.9% vs. 68.8%, p=0.023). VEGFA -2578, VEGFA -1154 and VEGFA +936 were significantly associated with infiltration to the pterygoid processes (p=0.011, p=0.041 and p=0.032, respectively). EDNRA H323H TT genotype was marginally associated with infiltration to the ipsilateral medial pterygoid muscles (p=0.045). A trend towards longer overall survival was observed for VEGFA -2578 CC as compared to AC (HR=0.24, p=0.060). The studied VEGFA SNPs seem to be associated with the local extension of the NPC and maybe with the clinical outcome of this patient group.