Inhibition of Type-2 Diabetes Mellitus Development by Sophocarpine through Targeting PPARy-Regulated Gene Expression

• Published in: Doklady. Biochemistry and biophysics Vol. 497; no. 1; pp. 137 - 143
• Main Authors: Su, Xiaoqian, Miao, Wei, Li, Li, Zheng, Haisheng, Hao, Gaimei, Du, Liang
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.03.2021; Springer Nature B.V
• Subjects: Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Ceruloplasmin; Diabetes; Diabetes mellitus; Gene expression; Hemoglobin; High density lipoprotein; Insulin; Life Sciences; Low density lipoprotein; Metabolic disorders; Molecular Biology; Oxidative stress; Pancreas; Peptides; Rodents; Vascular system; Vitamin E; sophocarpine; diabetes mellitus; streptozotocin; GSH; C-peptide
• ISSN: 1607-6729; 1608-3091
• DOI: 10.1134/S1607672921020150

Diabetes mellitus (DM), a metabolic disorder, is the causes of oxidative stress leading to complications in micro- and macro-vascular system. The present study investigated sophocarpine for anti-diabetic potential in vivo in mice model. Sophocarpine administration to diabetic mice significantly ( p < 0.05) attenuated glucose content in the plasma. The diabetes mediated lowering of GSH, ceruloplasmin and vitamin E was prevented in mice plasma by sophocarpine administration. Sophocarpine significantly ( p < 0.05) reversed diabetes mediated suppression of insulin level and total Hb content in the mice plasma. In sophocarpine administrated diabetic mice C-peptide level was elevated and glycosylated hemoglobin content was suppressed significantly ( p < 0.05) relative to diabetic group. Administration of sophocarpine significantly ( p  < 0.05) repressed diabetes mediated increase in TG and TC levels in dose-based manner. Administration of sophocarpine exhibited preventive role against diabetes mediated pathological damage to pancreas in the mice. Sophocarpine administration to diabetic mice repressed PPARγ recruitment significantly ( p < 0.05) in dose-dependent manner. Sophocarpine prevents oxidative stress mediated pancreatic damage through increase in vitamin E, GSH and C-peptide levels, Moreover, the PPARγ activity was down-regulated, LDL-c content lowered and HDL-c level elevated in diabetic mice by sophocarpine. Therefore, sophocarpine may be developed for treatment of diabetes, however, further in vivo studies need to confirm the same.