Marinobacter profundi sp. nov., a slightly halophilic bacterium isolated from a deep-sea sediment sample of the New Britain Trench

• Published in: Antonie van Leeuwenhoek Vol. 112; no. 3; pp. 425 - 434
• Main Authors: Cao, Junwei, Liu, Ping, Liu, Renju, Su, Hainan, Wei, Yuli, Liu, Rulong, Fang, Jiasong
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.03.2019; Springer Nature B.V
• Subjects: Bacteria; Base Composition; Biomedical and Life Sciences; Cardiolipin; Catalase; Cluster Analysis; Cytosol - chemistry; Deep sea; Deep sea environments; Deoxyribonucleic acid; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Enzymes - analysis; Fatty acids; Fatty Acids - analysis; Gene sequencing; Geologic Sediments - microbiology; Hydrogen-Ion Concentration; Hydrothermal Vents - microbiology; Life Sciences; Lipids; Marinobacter; Marinobacter - classification; Marinobacter - genetics; Marinobacter - isolation & purification; Marinobacter - physiology; Medical Microbiology; Microbiology; Multilocus Sequence Typing; New species; Original Paper; Pacific Ocean; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipids; Phospholipids - analysis; Phylogeny; Plant Sciences; Quinones; Quinones - analysis; RecA protein; RNA, Ribosomal, 16S - genetics; RpoB protein; rRNA 16S; Sediment samplers; Sequence Analysis, DNA; Sodium chloride; Sodium Chloride - metabolism; Soil Science & Conservation; Temperature; Ubiquinone; Pacific Ocean; Halophilic; New Britain Trench; Polyphasic taxonomy; Piezotolerant; Marinobacter profundi
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-018-1176-8

A piezotolerant, cold-adapted, slightly halophilic bacterium, designated strain PWS21 T , was isolated from a deep-sea sediment sample collected from the New Britain Trench. Cells were observed to be Gram-stain negative, rod-shaped, oxidase- and catalase-positive. Growth of the strain was observed at 4–45 °C (optimum 37 °C), at pH 5.0–9.0 (optimum 7.0) and in 0.5–20% (w/v) NaCl (optimum 3–4%). The optimum pressure for growth was 0.1 MPa (megapascal) with tolerance up to 70 MPa. 16S rRNA gene sequence analysis showed that strain PWS21 T is closely related to Marinobacter guineae M3B T (98.4%) and Marinobacter lipolyticus SM19 T (98.2%). Multilocus sequence analysis (MLSA) based on sequences of housekeeping genes gyrB , recA , atpD , rpoB and rpoD indicates that strain PWS21 T represents a distinct evolutionary lineage within the genus Marinobacter . Furthermore, strain PWS21 T showed low ANI and diDDH values to the closely related species. The principal fatty acids were identified as C 12:0 , C 12:0 3-OH, C 16:1 ω 9 c , C 16:0 and C 18:1 ω 9 c . Ubiquinone-9 was identified as the major respiratory quinone. The polar lipids were identified as phosphatidylethanolamine (PE), phosphatidylglycerol (PG), diphosphatidylglycerol (DPG), aminophospholipid (APL), two unidentified lipids and an unidentified phospholipid (PL). The G + C content of the genomic DNA was determined to be 60.3 mol%. On the basis of phenotypic, chemotaxonomic and molecular data, we conclude that strain PWS21 T represents a novel species of the genus Marinobacter , for which the name Marinobacter profundi sp. nov. is proposed (type strain PWS21 T  = KCTC 52990 T  = MCCC 1K03345 T ).