Molecular mapping of four blast resistance genes using recombinant inbred lines of 93-11 and nipponbare

• Published in: Journal of plant biology = Singmul Hakhoe chi Vol. 56; no. 2; pp. 91 - 97
• Main Authors: Yang, H.M., China Agricultural University, Beijing, China, Jia, M.H., Ohio State University, Columbus, OH, USA, Jia, Y.L., Ohio State University, Columbus, OH, USA, Xing, J.J., USDA-ARS Dale Bumpers National Rice Research Center, Stuttgart, USA, Venu,, USDA-ARS Dale Bumpers National Rice Research Center, Stuttgart, USA, R-C2, Ohio State University, Columbus, OH, USA, Bellizzi, M., Ohio State University, Columbus, OH, USA, Yuan, L., Ohio State University, Columbus, OH, USA, Wang, Z., Ohio State University, Columbus, OH, USA, Sun, C.Q., China Agricultural University, Beijing, China, Wang, G.L
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.04.2013; Springer Nature B.V; 한국식물학회
• Subjects: ARROZ; Biomedical and Life Sciences; Chromosome 1; Chromosome 11; Chromosome 12; Chromosomes; Cultivars; Disease resistance; Disease resistance gene,Magnaporthe oryzae,Nipponbare,,Rice blast; Gene mapping; Genes; Inbreeding; Life Sciences; Mapping; Marker-assisted selection; Molecular modelling; New varieties; Original Article; Phenotypic variations; Plant Breeding/Biotechnology; Plant Ecology; Plant Genetics and Genomics; Plant Sciences; Plant Systematics/Taxonomy/Biogeography; Quantitative trait loci; RICE; Rice blast; RIZ; 생물학; 9311; Nipponbare; Rice blast; Disease resistance gene
• ISSN: 1226-9239; 1867-0725
• DOI: 10.1007/s12374-012-0462-7

Molecular mapping of new blast resistance genes is important for developing resistant rice cultivars using marker-assisted selection. In this study, 259 recombinant inbred lines (RILs) were developed from a cross between Nipponbare and 93-11, and were used to construct a 1165.8-cM linkage map with 131 polymorphic simple sequence repeat (SSR) markers. Four major quantitative trait loci (QTLs) for resistance to six isolates of Magnaporthe oryzae were identified: qPi93-1, qPi93-2, qPi93-3, and qPiN-1. For the three genes identified in 93-11, qPi93-1 is linked with SSR marker RM116 on the short arm of chromosome 11 and explains 33% of the phenotypic variation in resistance to isolate CHE86. qPi93-2 is linked with SSR marker RM224 on the long arm of chromosome 11 and accounts for 31% and 25% of the phenotypic variation in resistance to isolates 162-8B and ARB50, respectively. qPi93-3 is linked with SSR marker RM7102 on chromosome 12 and explains 16%, 53%, and 28% of the phenotypic variation in resistance to isolates CHE86, ARB52, and ARB94, respectively. QTL qPiN-1 from Nipponbare is associated with SSR marker RM302 on chromosome 1 and accounts for 34% of the phenotypic variation in resistance to isolate PO6-6. These new genes can be used to develop new varieties with blast resistance via marker-aided selection and to explore the molecular mechanism of rice blast resistance.