Efficiencies of Generating Selectable Marker-Free Transgenic Rice with Different Transformation Methods

• Published in: Rice science Vol. 16; no. 4; pp. 254 - 260
• Main Authors: YU, Heng-xiu, LU, Mei-fang, CHEN, Xiu-hua, GONG, Zhi-yun, LIU, Qiao-quan, GU, Ming-hong
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.12.2009; Key Laboratory of Plant Functional Genomics of Ministry of Education/Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, Yangzhou University, Yangzhou 225009, China
• Subjects: Agrobacterium tumeficien; co-transformation; DNA载体; rice; selectable marker-free; 双元载体; 反义Wx基因; 地区组成; 水稻品种; 转化方法; 转基因水稻; 选择标记; rice; co-transformation; Agrobacterium tumeficien; selectable marker-free
• ISSN: 1672-6308; 1876-4762
• DOI: 10.1016/S1672-6308(08)60088-8

To study the efficiency of generating selectable marker-free （SMF） transgenic rice, two transformation methods were employed for four rice varieties （Wuxiangjing 9, Longtefu, Xieqingzao and Zhenshan 97）. One method is by using a single twin T-DNA binary vector pYH592 in one Agrobacterium strain, which is composed of two separate T-DNA regions （one carrying an antisense Wx gene and the other carrying a HPTgene）. The other one, named as two-strain/two-vector system, is by using two separate binary vectors in two separate Agrobacterium cultures. The results indicated that the average co-transformation frequencies of the antisense Wx gene and the HPT gene were 10.1% and 45.0%, respectively, for the four rice varieties. And the SMF transgenic plants selected from the offsprings of co-transformants were 55.6% and 60.0% in the two-strain/two-vector and twin T-DNA vector binary systems, respectively.