Identification and development of diagnostic markers for a powdery mildew resistance gene on chromosome 2R of Chinese rye cultivar Jingzhouheimai

• Published in: Molecular breeding Vol. 27; no. 4; pp. 455 - 465
• Main Authors: Zhuang, L. F, Sun, L, Li, A. X, Chen, T. T, Qi, Z. J
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.04.2011; Springer Netherlands; Springer Nature B.V
• Subjects: Airborne microorganisms; Alien chromosome line; Biomedical and Life Sciences; Biotechnology; Chromosomes; Cultivars; Diagnostic systems; Erysiphe graminis; Gene introgression; Gene mapping; Germplasm; Life Sciences; Mapping; Markers; Molecular biology; Plant biology; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Powdery mildew; Rye; Secale cereale; Substitution reactions; Triticum aestivum; Wheat; Gene introgression; Alien chromosome line
• ISSN: 1380-3743; 1572-9788
• DOI: 10.1007/s11032-010-9443-z

Chinese rye cultivar Jingzhouheimai (Secale cereale L.) shows a high level of resistance to powdery mildew. Identification, location, and mapping of the resistance gene would be helpful for developing a highly resistant germplasm or cultivar in wheat. Using sequential C-banding, GISH, and marker analysis, an addition chromosome with powdery mildew resistance was identified in a line derived from a cross between Chinese wheat landrace Huixianhong and rye cultivar Jingzhouheimai. The line, designated H-J DA2RDS1R(1D), had 44 chromosomes including two pairs of rye chromosomes, 1R and 2R, and lacked a pair of wheat chromosomes 1D, that is, it is a double disomic addition disomic substitution line. According to its reaction to different isolates of the powdery mildew pathogen, the resistance gene in H-J DA2RDS1R(1D) differed from the Pm8 and Pm7 genes located earlier on rye chromosomes 1R and 2R, respectively. In order to determine the location of the resistance gene, line H-J DA2RDS1R(1D) was crossed with wheat landrace Huixianhong and the F2 population and corresponding F2:3 families were tested for disease reaction and assessed with molecular markers. The results showed that a resistance gene, designated PmJZHM2RL, is located in rye chromosome arm 2RL.