Transparent diamond microelectrodes for biochemical application

• Published in: Diamond and related materials Vol. 19; no. 7; pp. 1021 - 1026
• Main Authors: Gao, Z., Carabelli, V., Carbone, E., Colombo, E., Demaria, F., Dipalo, M., Gosso, S., Manfredotti, Ch, Pasquarelli, A., Rossi, S., Xu, Y., Vittone, E., Kohn, E.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 01.07.2010; Elsevier
• Subjects: Arrays; Beneficiation; Chemical vapor deposition (including plasma-enhanced cvd, mocvd, etc.); Chemistry; Cross-disciplinary physics: materials science; rheology; Diamond; Electrochemistry; Electrodes; Electrodes: preparations and properties; Exact sciences and technology; Filaments; Fullerenes and related materials; diamonds, graphite; General and physical chemistry; Materials science; MEA; Methods of deposition of films and coatings; film growth and epitaxy; Microelectrodes; Nanoscale materials and structures: fabrication and characterization; Nanostructure; Other topics in nanoscale materials and structures; Physics; Recording; Sapphire; Specific materials; Transparency; MEA; Transparency; Diamond; Hot filament chemical vapor deposition; Microelectrodes; Nucleation; Synthetic diamond; Carbon electrode; Fluorescence; Doped materials; Thin films; Polycrystalline diamond; Electrochemical properties; Optical properties; Boron additions; Oxidation; Beryllium nitride; Arrays; Nanostructured materials
• ISSN: 0925-9635; 1879-0062
• DOI: 10.1016/j.diamond.2010.03.014

In this investigation a technology has been developed to use diamond electrodes in Micro Electrode Arrays (MEAs) on a transparent sapphire substrate, thus combining the outstanding electrochemical properties of boron-doped diamond electrodes (BDD) with the transparency needed for simultaneous fluorescence analysis. Nanodiamond films were grown on double side polished sapphire substrates by hot filament CVD (HFCVD) and Bias Enhanced Nucleation (BEN). A simple four microelectrode array (quadrupole) has been fabricated, fully characterized in optical and electrochemical properties, and tested with adrenal chromaffin cells, identifying amperometric spikes by the four recording diamond electrodes, corresponding to the oxidation current of catecholamine molecules.