Imidacloprid impedes mitochondrial function and induces oxidative stress in cotton bollworm, Helicoverpa armigera larvae (Hubner: Noctuidae)
Neonicotinoids have high agonistic affinity to insect nicotinic acetylcholine receptors (nAChR) and are frequently used as insecticides against most devastating lepidopteran insect pests. Imidacloprid influenced dose-dependent decline in the state III and IV respiration, respiration control index (R...
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Published in | Journal of bioenergetics and biomembranes Vol. 50; no. 1; pp. 21 - 32 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
New York
Springer US
01.02.2018
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Neonicotinoids have high agonistic affinity to insect nicotinic acetylcholine receptors (nAChR) and are frequently used as insecticides against most devastating lepidopteran insect pests. Imidacloprid influenced dose-dependent decline in the state III and IV respiration, respiration control index (RCI), and
P/O
ratios, in vitro and in vivo. The bioassay indicated its LD
50
value to be 531.24 μM. The insecticide exhibited a dose-dependent inhibition on F
0
F
1
-ATPase and complex IV activity. At 600 μM, the insecticide inhibited 83.62 and 27.13% of F
0
F
1
-ATPase and complex IV activity, respectively, and induced the release of 0.26 nmoles/min/mg protein of cytochrome c. A significant dose- and time-dependent increase in oxidative stress was observed; at 600 μM, the insecticide correspondingly induced lipid peroxidation, LDH activity, and accumulation of H
2
O
2
content by 83.33, 31.51 and 223.66%. The stress was the maximum at 48 h of insecticide treatment (91.58, 35.28, and 189.80%, respectively). In contrast, catalase and superoxide dismutase were reduced in a dose- and time-dependent manner in imidacloprid-fed larvae. The results therefore suggest that imidacloprid impedes mitochondrial function and induces oxidative stress in
H. armigera
, which contributes to reduced growth of the larvae along with its neurotoxic effect. |
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ISSN: | 0145-479X 1573-6881 |
DOI: | 10.1007/s10863-017-9739-3 |