Changes of Inhibitory Receptors on NK-92 Cells and HLA-G on BeWo Cells with Toxoplasma gondii Infection

• Published in: Inflammation Vol. 36; no. 6; pp. 1440 - 1447
• Main Authors: Liu, Yang, Zhang, Ling, Gao, Meilan, Zhang, Fengmei, Xu, Xiaoyan, Liu, Xianbing, Hu, Xuemei
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.12.2013; Springer Nature B.V
• Subjects: Antigens, CD - metabolism; Biomedical and Life Sciences; Biomedicine; Cell Line; Coculture Techniques; Female; HLA-G Antigens - metabolism; Humans; Immunology; Internal Medicine; Killer Cells, Natural - immunology; Leukocyte Immunoglobulin-like Receptor B1; Pathology; Pharmacology/Toxicology; Pregnancy; Pregnancy Complications - immunology; Pregnancy Complications - parasitology; Receptors, Immunologic - metabolism; Receptors, KIR2DL4 - metabolism; Rheumatology; Toxoplasma - immunology; Toxoplasmosis - immunology; adverse pregnancy outcome; HLA-G; KIR2DL4; ILT-2
• ISSN: 0360-3997; 1573-2576
• DOI: 10.1007/s10753-013-9684-1

The aim of this study were to demonstrate the effects of Yellow fluorescent protein- Toxoplasma gondii infection on the NK-92 cells co-cultured with BeWo cells in vitro and to verify the implications upon adverse pregnancy outcome. There are four groups including NK-92 cells infected or uninfected with T. gondii , and NK-92 cells co-cultured with BeWo cells in the presence or absent of T. gondii infection. Cells were observed by fluorescence microscope at 12, 24, 36, 48, and 60 h after infection. Levels of inhibitory receptors killer immunoglobulin-like receptor (KIR)2DL4 and ILT-2 expressed on NK-92 cells, and their common ligand HLA-G expressed on BeWo cells were measured by real-time PCR and flow cytometry in all groups. Levels of KIR2DL4, ILT-2 and HLA-G increased at 12, 24, 36, and 48 h, while decreased at 60 h in all T. gondii -infected groups compared with their parallel control groups, respectively. KIR2DL4 and ILT-2 expression in co-culture groups with infection were higher than those in infected NK-92 groups at 12, 24, 36, and 48 h, while there were no significant differences at 60 h. The expression of the two inhibitory receptors was correlated with HLA-G expression in infected co-culture groups. The changes of inhibitory receptors on NK-92 cells and their common ligand HLA-G on BeWo cells with T. gondii infection might contribute to the occurrence of adverse pregnancy outcome.