Expression of intercellular adhesion molecule-1 (ICAM-1) during ischemia-reperfusion in human liver tissue allograft: Image analysis by confocal laser scanning microscopy

• Published in: Digestive diseases and sciences Vol. 48; no. 11; pp. 2167 - 2172
• Main Authors: BENKOEL, Liliane, DODERO, Frank, HARDWIGSEN, Jean, BENOLIEL, Anne Marie, BONGRAND, Pierre, BOTTA-FRIDLUND, Danielle, LE TREUT, Yves Patrice, CHAMLIAN, Albert, LOMBARDO, Dominique
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.11.2003; Springer Nature B.V
• Subjects: Adult; Biological and medical sciences; Cell Membrane - metabolism; Endothelium - metabolism; Female; Hepatocytes - metabolism; Humans; Image Processing, Computer-Assisted; Intercellular Adhesion Molecule-1 - metabolism; Liver - metabolism; Liver - pathology; Liver Transplantation - adverse effects; Liver, biliary tract, pancreas, portal circulation, spleen; Male; Medical sciences; Microscopy, Confocal; Middle Aged; Reperfusion Injury - metabolism; Reperfusion Injury - pathology; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the digestive system; Human; Endothelial cell; Hepatic sinusoid; Intercellular adhesion molecule 1; Digestive system; Liver; Cardiovascular disease; Confocal microscopy; Transplantation; ICAM-1; human liver allografts; ischemia-reperfusion; Homotransplantation; Biological activity; Reperfusion; Ischemia; Surgery; confocal laser scanning microscopy
• ISSN: 0163-2116; 1573-2568
• DOI: 10.1023/B:DDAS.0000004521.88660.fd

We studied and quantified the effect of ischemia-reperfusion on expression of intercellular adhesion molecule-1 (ICAM-1) on sinusoidal endothelial cells and hepatocyte plasma membranes by means of confocal laser scanning microscopy imaging. We found that ischemia induced an increase in ICAM-1 expression on sinusoidal endothelial cells and hepatocytes. After reperfusion, ICAM-1 expression was increased on sinusoidal endothelial cells, whereas it was unmodified on hepatocytes. On the other hand, ICAM-1 expression was not correlated to ischemia-reperfusion liver injury. Therefore, hepatocellular ischemia-reperfusion injury could be induced by other causes than immune-mediated damages.