Aestuarium zhoushanense gen. nov., sp. nov., Isolated from the Tidal Flat

• Published in: Current microbiology Vol. 74; no. 12; pp. 1469 - 1476
• Main Authors: Yu, Xiao-Yun, Zhai, Jing-Yu, Fu, Ge-Yi, Shen, Xia, Zhao, Zhe, Wu, Min, Zhang, Xin-Qi
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.12.2017; Springer Nature B.V
• Subjects: Aerobiosis; Aestuarium zhoushanense; Bacterial Typing Techniques; Base Composition; Biomedical and Life Sciences; Biotechnology; China; Cluster Analysis; Coasts; Cytosol - chemistry; Deoxyribonucleic acid; DNA; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Environmental Microbiology; Fatty acids; Fatty Acids - analysis; Gene sequencing; Gram-negative bacilli; Hydrogen-Ion Concentration; Lecithin; Life Sciences; Lipids; Locomotion; Microbiology; Microscopy, Electron, Transmission; New genera; New species; pH effects; Phosphatidylcholine; Phosphatidylethanolamine; Phosphatidylglycerol; Phospholipids - analysis; Phylogenetics; Phylogeny; Physical characteristics; Quinones; Quinones - analysis; Rhodobacteraceae; RNA, Ribosomal, 16S - genetics; Roseobacter - classification; Roseobacter - genetics; Roseobacter - isolation & purification; Roseobacter - physiology; rRNA 16S; Sequence Analysis, DNA; Sodium chloride; Sodium Chloride - metabolism; Strain rate; Temperature; Tidal flats; Tidal waves
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-017-1342-7

A gram-stain-negative, aerobic, ovoid or short rod-shaped, and non-motile strain, designed G7 T was isolated from a tidal flat sample collected from the coast of East Sea in Zhoushan, China. Strain G7 T grew at 4–40 °C and pH 6.0–9.0 (optimum, 28 °C and pH 7.5) and with 0–7% (w/v) NaCl (optimum, 1%). The predominant respiratory quinone was Q-10 and the major fatty acids (>10%) identified were C 18:1 ω 7 c , C 16:0 and summed feature 3 (C 16:1 ω 7 c and/or C 16:1 ω 6 c ). The polar lipids of strain G7 T consisted of phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, and four unidentified lipids. The genomic DNA G+C content was 56.7 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain G7 T formed a distinct lineage belonging to the Roseobacter clade of the family Rhodobacteraceae . On the basis of morphological, physiological, and chemotaxonomic characteristics, together with the results of phylogenetic analysis, strain G7 T is described as a novel species in a new genus, for which the name Aestuarium zhoushanense gen. nov., sp. nov. (type strain G7 T  = MCCC 1K03229 T  = KCTC 52584 T ) is proposed.