Sphingomonas ginsenosidivorax sp. nov., with the ability to transform ginsenosides

• Published in: Antonie van Leeuwenhoek Vol. 103; no. 6; pp. 1359 - 1367
• Main Authors: Jin, Xue-Feng, Kim, Jin-Kwang, Liu, Qing-Mei, Kang, Myung-Suk, He, Dan, Jin, Feng-Xie, Kim, Sun-Chang, Im, Wan-Taek
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.06.2013; Springer Nature B.V
• Subjects: Bacterial Typing Techniques; Base Composition; Base Sequence; Biomedical and Life Sciences; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Fatty Acids; Fresh Water - microbiology; Genes, rRNA; Geologic Sediments - microbiology; Ginsenosides - metabolism; Life Sciences; Medical Microbiology; Microbiology; Molecular Sequence Data; Original Paper; Phylogenetics; Phylogeny; Plant Sciences; Polyamines - metabolism; Republic of Korea; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil Microbiology; Soil Science & Conservation; Sphingomonas - classification; Sphingomonas - genetics; Sphingomonas - isolation & purification; Sphingomonas - metabolism; Taxonomy; Republic of Korea; Ginsenoside; Polyphasic taxonomy; 16S rRNA gene
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-013-9916-2

A Gram-negative, strictly aerobic, non-motile, non-spore-forming and rod-shaped bacterial strain designated KHI67 T was isolated from sediment of the Gapcheon River in South Korea and its taxonomic position was investigated by using a polyphasic approach. Strain KHI67 T was observed to grow optimally at 25–30 °C and at pH 7.0 on nutrient and R2A agar. On the basis of 16S rRNA gene sequence similarity, strain KHI67 T was shown to belong to the family Sphingomonadaceae and was related to Sphingomonas faeni MA-olki T (97.6 % sequence similarity), Sphingomonas aerolata NW12 T (97.5 %) and Sphingomonas aurantiaca MA101b T (97.3 %). The G + C content of the genomic DNA was determined to be 65.6 %. The major ubiquinone was found to be Q-10, the major polyamine was identified as homospermidine and the major fatty acids identified were summed feature 8 (comprising C 18:1 ω 7 c / ω 6 c ; 37.0 %), C 16:0 (13.0 %), summed feature 3 (comprising C 16:1 ω 7 c /C 16:1 ω 6 c ; 12.8 %) and C 14:0 2OH (9.3 %). DNA and chemotaxonomic data supported the affiliation of strain KHI67 T to the genus Sphingomonas . The DNA–DNA relatedness values between strain KHI67 T and its closest phylogenetic neighbours were below 15 %. Strain KHI67 T could be differentiated genotypically and phenotypically from the recognised species of the genus Sphingomonas . The isolate therefore represents a novel species, for which the name Sphingomonas ginsenosidivorax sp. nov. is proposed, with the type strain KHI67 T (=KACC 14951 T  = JCM 17076 T  = LMG 25801 T ).