Enzyme-linked immunosorbent assay and agar gel immunodiffusion assay for diagnosis of equine infectious anemia employing p26 protein fused to the maltose-binding protein

• Published in: Archives of virology Vol. 163; no. 10; pp. 2871 - 2875
• Main Authors: Fontes, Karin F. L. P., Silva-Júnior, Luiz C., Nascimento, Sérgio A., Chaves, Daniel P., Pinheiro-Júnior, Jose W., Freitas, Antonio C., Castro, Roberto S., Jesus, André L. S.
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.10.2018; Springer Nature B.V
• Subjects: Anemia; Antigens; Biomedical and Life Sciences; Biomedicine; Brief Report; Cross-reaction; Diagnosis; Enzyme-linked immunosorbent assay; Enzymes; Equine infectious anemia; Horses; Immunodiffusion; Immunoreactivity; Infectious Diseases; Maltose; Maltose-binding protein; Medical Microbiology; p26 Protein; Proteins; Vaccination; Virology
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-018-3923-6

A codon-optimized equine infectious anemia virus p26 gene was fused to a maltose-binding protein (MBP) and expressed in Escherichia coli for use as an antigen in agar gel immunodiffusion (AGID) and enzyme-linked immunosorbent assay (ELISA) for diagnosis of equine infectious anemia. An analysis of analytical sensitivity and specificity showed that the antigen MBP-p26rec reacted positively with a reference World Organization for Animal Health serum and demonstrated no cross-reaction against sera from vaccinated animals in either test. The diagnostic characteristics were evaluated and presented excellent values. The AGIDrec showed 100% sensitivity and specificity, and the ELISArec showed 100% sensitivity and 99.64% specificity. In addition, MBP-p26rec was stabile after three years of storage at 4 °C, maintaining its immunoreactivity.