Dark-pigmented biodeteriogenic fungi in etruscan hypogeal tombs: New data on their culture-dependent diversity, favouring conditions, and resistance to biocidal treatments

• Published in: Fungal biology Vol. 125; no. 8; pp. 609 - 620
• Main Authors: Isola, Daniela, Zucconi, Laura, Cecchini, Adele, Caneva, Giulia
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.08.2021
• Subjects: benzalkonium chloride; biocides; Black fungi; Cladosporiaceae; cultural heritage; Exophiala; fungi; humidity; light intensity; Metabolic screening; microclimate; mycology; new taxa; Quaternary ammonium salts; risk; Subterranean sites; Wall paintings; Wall paintings; Quaternary ammonium salts; Subterranean sites; Metabolic screening; Black fungi
• ISSN: 1878-6146; 1878-6162
• DOI: 10.1016/j.funbio.2021.03.003

Subterranean Cultural Heritage sites are frequently subject to biological colonization due to the high levels of humidity, even in conditions of low irradiance and oligotrophy. Here microorganisms form complex communities that may be dangerous through mineral precipitation, through the softening of materials or causing frequent surface discolorations. A reduction of contamination’s sources along with the control of microclimatic conditions and biocide treatments (overall performed with benzalkonium chloride) are necessary to reduce microbial growths. Dark discolorations have been recorded in the painted Etruscan tombs of Tarquinia, two of which have been analyzed to collect taxonomical, physiological, and ecological information. Eighteen dark-pigmented fungi were isolated among a wider culturable fraction: nine from blackening areas and nine from door sealings, a possible route of contamination. Isolates belonged to three major groups: Chaetothyriales, Capnodiales (Family Cladosporiaceae), and Acremonium-like fungi. Exophiala angulospora and Cyphellophora olivacea, a novelty for hypogea, were identified, while others need further investigations as possible new taxa. The metabolic skills of the detected species showed their potential dangerousness for the materials. Their tolerance to benzalkonium chloride-based products suggested a certain favouring effect through the decreasing competitiveness of less resistant species. The type of covering of the dromos may influence the risk of outer contamination. Fungal occurrence can be favoured by root penetration. •Tolerance to biocides affects fungal spreading.•Metabolic screenings show fungal dangerousness for materials.•Subterranean environments show unknown fungal taxa.