The 5-oxoprolinase is required for conidiation, sexual reproduction, virulence and deoxynivalenol production of Fusarium graminearum
In eukaryotic organisms, the 5-oxoprolinase is one of the six key enzymes in the γ-glutamyl cycle that is involved in the biosynthetic pathway of glutathione (GSH, an antioxidative tripeptide counteracting the oxidative stress). To date, little is known about the biological functions of the 5-oxopro...
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Published in | Current genetics Vol. 64; no. 1; pp. 285 - 301 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.02.2018
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | In eukaryotic organisms, the 5-oxoprolinase is one of the six key enzymes in the γ-glutamyl cycle that is involved in the biosynthetic pathway of glutathione (GSH, an antioxidative tripeptide counteracting the oxidative stress). To date, little is known about the biological functions of the 5-oxoprolinase in filamentous phytopathogenic fungi. In this study, we investigated the 5-oxoprolinase in
Fusarium graminearum
for the first time. In
F. graminearum,
two paralogous genes (
FgOXP1
and
FgOXP2
) were identified to encode the 5-oxoprolinase while only one homologous gene encoding the 5-oxoprolinase could be found in other filamentous phytopathogenic fungi or
Saccharomyces cerevisiae
. Deletion of
FgOXP1
or
FgOXP2
in
F. graminearum
led to significant defects in its virulence on wheat. This is likely caused by an observed decreased deoxynivalenol (DON, a mycotoxin) production in the gene deletion mutant strains as DON is one of the best characterized virulence factors of
F. graminearum
. The
FgOXP2
deletion mutant strains were also defective in conidiation and sexual reproduction while the
FgOXP1
deletion mutant strains were normal for those phenotypes. Double deletion of
FgOXP1
and
FgOXP2
led to more severe defects in conidiation, DON production and virulence on plants, suggesting that both
FgOXP1
and
FgOXP2
play a role in fungal development and plant colonization. Although transformation of
MoOXP1
into
ΔFgoxp1
was able to complement
ΔFgoxp1
, transformation of
MoOXP1
into
ΔFgoxp2
failed to restore its defects in sexual development, DON production and pathogenicity. Taken together, these results suggest that
FgOXP1
and
FgOXP2
are likely to have been functionally diversified and play significant roles in fungal development and full virulence in
F. graminearum. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0172-8083 1432-0983 |
DOI: | 10.1007/s00294-017-0747-y |