The Effect of Low and Medium Doses of Proton Pencil Scanning Beam on the Blood-Forming Organs during Total Irradiation of Mice

• Published in: Doklady. Biochemistry and biophysics Vol. 494; no. 1; pp. 231 - 234
• Main Authors: Balakin, V. E., Rozanova, O. M., Smirnova, E. N., Belyakova, T. A., Shemyakov, A. E., Zaichkina, S. I., Sorokina, S. S., Dyukina, A. R.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01.09.2020; Springer Nature B.V
• Subjects: Animals; Biochemistry; Biological and Medical Physics; Biomedical and Life Sciences; Biophysics; Blood cells; Bone marrow; Bone Marrow - metabolism; Bone Marrow - pathology; Bone Marrow - radiation effects; Cytogenetics; Life Sciences; Lymphoid Tissue - metabolism; Lymphoid Tissue - pathology; Lymphoid Tissue - radiation effects; Male; Mice; Molecular Biology; Proton Therapy - adverse effects; Proton Therapy - methods; Radiation Dosage; Reactive oxygen species; Reactive Oxygen Species - metabolism; Relative Biological Effectiveness; Scanning; Whole-Body Irradiation - adverse effects; Whole-Body Irradiation - methods; thymus; spleen; ROS; micronucleus test; pencil beam scanning; mice; proton
• ISSN: 1607-6729; 1608-3091
• DOI: 10.1134/S1607672920050026

The aim of this work was to study the effect of proton pencil beam scanning in the Bragg peak in the dose range of 0.1–1.5 Gy on the induction of cytogenetic damage in the bone marrow, reactive oxygen species (ROS) production in whole blood, and the state of lymphoid organs after total body irradiation of mice. Irradiation was carried out in the Prometeus proton synchrotron (Protvino) in the Bragg peak with proton energy at the output of 90–116 MeV. It was found that, under irradiation of mice in the range of low and medium doses of proton pencil beam scanning in the Bragg peak, the relative biological effectiveness (RBE) according to the criterion of cytogenetic changes was 1.15. In addition, it was found that the pathophysiological effect on the lymphoid organs and the production of ROS by blood cells were different as compared with the effect of X-rays.