Zinc and Selenium Co-supplementation Reduces Some Lipid Peroxidation and Angiogenesis Markers in a Rat Model of NAFLD-Fed High Fat Diet

• Published in: Biological trace element research Vol. 181; no. 2; pp. 288 - 295
• Main Authors: Mousavi, Seyedeh Neda, Faghihi, Amirhosein, Motaghinejad, Majid, Shiasi, Maryam, Imanparast, Fatemeh, Amiri, Hamid Lorvand, Shidfar, Farzad
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.02.2018; Springer Nature B.V
• Subjects: Angiogenesis; Animal diseases; Animals; Biochemistry; Biomarkers - metabolism; Biomedical and Life Sciences; Biotechnology; Calories; Cardiovascular diseases; Cardiovascular Diseases - prevention & control; Diet; Diet, High-Fat; Dietary Supplements; Disease Models, Animal; Diseases; Fatty liver; Growth factors; High density lipoprotein; High fat diet; Insulin; Life Sciences; Lipid peroxidation; Lipid Peroxidation - drug effects; Lipids; Liver; Liver diseases; Low density lipoprotein; Male; Malondialdehyde; Markers; Neovascularization, Pathologic - drug therapy; Non-alcoholic Fatty Liver Disease - drug therapy; Nutrient deficiency; Nutrition; Oncology; Patients; Peroxidation; Rats; Rats, Sprague-Dawley; Rodents; Selenium; Selenium - administration & dosage; Selenium - pharmacology; Serum; Vascular endothelial growth factor; Zinc; Zinc - administration & dosage; Zinc - pharmacology; Lipid peroxidation; Angiogenesis; Cardiovascular disease; Supplementation; Non-alcoholic fatty liver disease
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-017-1059-2

Studies have shown that non-alcoholic fatty liver disease (NAFLD) patients are more prone to cardiovascular disease (CVD). Zinc and selenium deficiency are common in NAFLD. But the effects of zinc and selenium co-supplementation before and/or after disease progression on CVD markers are not clear in NAFLD patients. This study aimed to compare the effects of zinc and selenium co-supplementation before and/or after disease progression on some of the CVD markers in an experimental model of NAFLD. Forty male Sprague Dawley rats (197 ± 4 g) were randomly assigned into four dietary groups: control group (C; received 9% of calorie as fat), model group (M; received 82% of calorie as fat), and supplementation before (BS) or after (AS) disease progression. Animals were fed diets for 20 weeks in all groups. Fasting plasma glucose (FPG), insulin, HOMA-IR, ALT, AST, lipid profile, malondialdehyde (MDA) and vascular endothelial growth factor (VEGF) levels were measured as CVD indices. Serum ALT, AST, FPG, insulin, MDA, VEGF and HOMA-IR were significantly higher in the M than C group. Co-supplementation reduced serum ALT and AST levels in the BS and AS groups compared with the M group. FPG, insulin, HOMA-IR, VEGF, MDA, LDL/HDL-c and TC/HDL-c ratio were significantly reduced in the AS compared with the M group. TG/HDL-c ratio was significantly reduced in the BS and AS compared with the M group. Serum MDA, VEGF, Insulin and HOMA-IR were significantly lowered in the AS than BS group ( p  < 0.05). Zinc and selenium co-supplementation after NAFLD progression reduced CVD risk indices in an experimental model.