Volatile organic compounds produced by Bacillus velezensis L1 as a potential biocontrol agent against postharvest diseases of wolfberry
Volatile organic compounds (VOCs) produced by antagonistic microorganisms have good biocontrol prospects against postharvest diseases. Infection caused by Alternaria iridiaustralis and 10 other significant fungal diseases can be successfully inhibited by VOCs produced by an identified and screened e...
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Published in | Frontiers in microbiology Vol. 13; p. 987844 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
24.08.2022
|
Subjects | |
Online Access | Get full text |
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Summary: | Volatile organic compounds (VOCs) produced by antagonistic microorganisms have good biocontrol prospects against postharvest diseases. Infection caused by
Alternaria iridiaustralis
and 10 other significant fungal diseases can be successfully inhibited by VOCs produced by an identified and screened endophytic strain L1 (
Bacillus velezensis
). This study revealed the
in vivo
and
in vitro
biocontrol effects of VOCs released by
B. velezensis
L1 on
A. iridiaustralis
, a pathogenic fungus responsible for rot of wolfberry fruit. The inhibition rates of VOCs of
B. velezensis
L1 on the mycelial growth of
A. iridiaustrali
s
in vitro
were 92.86 and 90.30%, respectively, when the initial inoculum concentration on the plate was 1 × 10
9
colony forming unit (CFU)/ml. Spore germination and sporulation were 66.89 and 87.96%, respectively. VOCs considerably decreased the wolfberry’s disease index and decay incidence
in vivo
. Scanning electron microscopy revealed that the morphological and structural characteristics of
A. iridiaustralis
could be altered by VOCs. Ten VOCs were identified through headspace-gas chromatography-ion mobility spectrometry. Pure chemical tests revealed that 2.3-butanedione had the strongest antifungal effects, totally inhibiting
A. iridiaustralis
in wolfberry fruit at a 60 μl/L concentration. The theory underpinning the potential application of VOCs from
B. velezensis
is provided herein. This is also the first study to document the antifungal capabilities of the
B. velezensis
strain on postharvest wolfberry fruit.
GRAPHICAL ABSTRACT |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Elsherbiny A. Elsherbiny, Mansoura University, Egypt; Sheng Qin, Jiangsu Normal University, China This article was submitted to Food Microbiology, a section of the journal Frontiers in Microbiology Edited by: Khamis Youssef, Agricultural Research Center, Egypt |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2022.987844 |