CaHSP16.4, a small heat shock protein gene in pepper, is involved in heat and drought tolerance
Environmental stress affects growth and development of crops, and reduces yield and quality of crops. To cope with environmental stressors, plants have sophisticated defense mechanisms, including the HSF/HSP pathway. Here, we identify the expression pattern of CaHSP16.4 in thermo-tolerant and thermo...
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Published in | Protoplasma Vol. 256; no. 1; pp. 39 - 51 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Vienna
Springer Vienna
01.01.2019
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Environmental stress affects growth and development of crops, and reduces yield and quality of crops. To cope with environmental stressors, plants have sophisticated defense mechanisms, including the HSF/HSP pathway. Here, we identify the expression pattern of
CaHSP16.4
in thermo-tolerant and thermo-sensitive pepper (
Capsicum annuum
L.) lines
.
Under heat stress, R9 thermo-tolerant line had higher
CaHSP16.4
expression level than the B6 thermo-sensitive line. Under drought stress, expression pattern of
CaHSP16.4
was dynamic. Initially,
CaHSP16.4
was downregulated then
CaHSP16.4
significantly increased. Subcellular localization assay showed that
CaHSP16.4
localizes in cytoplasm and nucleus. In the R9 line, silencing of
CaHSP16.4
resulted in a significant increase in malonaldehyde content and a significant reduction in total chlorophyll content, suggesting that silencing of
CaHSP16.4
reduces heat and drought stresses tolerance. Overexpression of
CaHSP16.4
enhances tolerance to heat stress in
Arabidopsis
. Under heat stress, the survival rate of
CaHSP16.4
overexpression lines was significantly higher than wild type. Furthermore, under heat, drought, and combined stress conditions, the
CaHSP16.4
-overexpression lines had lower relative electrolytic leakage and malonaldehyde content, higher total chlorophyll content, and higher activity levels of superoxide dismutase, catalase, ascorbic acid peroxidase, and glutathione peroxidase compared to wild type. Furthermore, the expression levels of the stress response genes in the overexpression lines were higher than the wild type. These results indicate that the overexpression of
CaHSP16.4
enhances the ability of reactive oxygen species scavenging under heat and drought stress. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0033-183X 1615-6102 |
DOI: | 10.1007/s00709-018-1280-7 |