Molecular study of human sperm RNA: Ropporin and CABYR in asthenozoospermia

• Published in: Journal of endocrinological investigation Vol. 41; no. 7; pp. 781 - 787
• Main Authors: Pelloni, M., Paoli, D., Majoli, M., Pallotti, F., Carlini, T., Lenzi, A., Lombardo, F.
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.07.2018; Springer Nature B.V
• Subjects: Adolescent; Adult; Asthenozoospermia - genetics; Asthenozoospermia - pathology; Calcium; Calcium-Binding Proteins - analysis; Calcium-Binding Proteins - genetics; Endocrinology; Flagella; Gene expression; Humans; Male; Medicine; Medicine & Public Health; Membrane Proteins - analysis; Membrane Proteins - genetics; Metabolic Diseases; Middle Aged; Motility; Original Article; Phosphoproteins - analysis; Phosphoproteins - genetics; Phosphorylation; rho GTP-Binding Proteins - analysis; rho GTP-Binding Proteins - genetics; RNA - analysis; RNA - metabolism; Semen; Semen Analysis; Sequence Analysis, RNA; Sperm; Sperm Motility - genetics; Sperm Tail - physiology; Spermatogenesis; Spermatozoa - chemistry; Spermatozoa - metabolism; Spermatozoa - pathology; Tyrosine; Young Adult; CABYR; Ropporin; Asthenozoospermia; Human sperm mRNA
• ISSN: 1720-8386; 0391-4097; 1720-8386
• DOI: 10.1007/s40618-017-0804-x

Background Sperm motility is an essential aspect of human fertility. Sperm contain an abundance of transcripts, thought to be remnants of mRNA, which comprise a genetic fingerprint and can be considered a historic record of gene expression during spermatogenesis. The aberrant expression of numerous genes has been found to contribute to impaired sperm motility; these include ROPN1 (rhophilin associated tail protein 1), which encodes a component of the fibrous sheath of the mammalian sperm flagella, and CABYR (calcium-binding tyrosine-(Y)-phosphorylation-regulated protein), which plays an important role in calcium activation and modulation. The aim of this study was to investigate ROPN1 and CABYR gene co-expression in asthenozoospermic semen samples in comparison with normozoospermic samples. Methods We studied 120 semen samples (60 normozoospermic and 60 asthenozoospermic) from Caucasian patients attending our centre for an andrological check-up. Total RNA was extracted from purified spermatozoa with RNeasy mini kit. ROPN1 and CABYR mRNA expression was analysed using RT-qPCR. Continuous variables were described as means ± standard deviations. Results ROPN1 and CABYR mRNA were simultaneously downregulated in asthenozoospermic in comparison with normozoospermic samples. There was also a positive correlation between total progressive motility and ROPN1 and CABYR gene expression and between total motile sperm number and ROPN1 and CABYR gene expression. Conclusions The results demonstrated downregulation of both ROPN1 and CABYR in asthenozoospermic samples and importantly, a positive correlation between the expression of the two genes, suggesting that ROPN1 and CABYR co-expression is a prerequisite for normal flagellar function and sperm motility.