Proteomic analysis of A-549 cells infected with human adenovirus 40 by LC-MS

• Published in: Virus genes Vol. 54; no. 3; pp. 351 - 360
• Main Authors: Guissoni, Ana Carla Peixoto, Soares, Célia Maria Almeida, Badr, Kareem R., Ficcadori, Fabiola Sousa, Parente, Ana Flávia Alves, Parente, Juliana Alves, Baeza, Lilian Cristina, Souza, Menira, Cardoso, Divina das Dores de Paula
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.06.2018; Springer Nature B.V
• Subjects: Adenoviruses; Apoptosis; Biomedical and Life Sciences; Biomedicine; Cell culture; Cellular stress response; Cystitis; Cytoskeleton; Electron transport; Gastroenteritis; Glycolysis; Homeostasis; Infections; Medical Microbiology; Metabolic pathways; Oxidation; Plant Sciences; Proteins; Respiratory diseases; Virology; A-549 cells; Human adenovirus; Proteomic analyses
• ISSN: 0920-8569; 1572-994X
• DOI: 10.1007/s11262-018-1554-3

Human Adenoviruses (HAdVs) are etiological agents of different syndromes such as gastroenteritis, cystitis, ocular, and respiratory diseases, and infection by these viruses may cause alterations in cellular homeostasis. The objective of the study was the proteomic analysis of A-549 cells infected with HAdV-40 using LC–MS. At 30 h of infection, the quantitative analysis revealed 336 differentially expressed proteins. From them, 206 were induced (up-regulated) and 130 were suppressed (down-regulated). The majority of up-regulated proteins were related to energy, cellular organization, stress response, and apoptosis pathways. It was observed alteration of cell metabolism with increase of the glycolytic pathway, β-oxidation, and respiratory chain. Also, the results suggest cytoskeleton reorganization and apoptosis induction. The data can improve knowledge about the replication of HAdV-40 in cell culture considering the proteins related to distinct metabolic pathways induced by viral infection in A-549 cells.